Growth rate maximization is an important fitness strategy for microbes. However, the wide
distribution of slow-growing oligotrophic microbes in ecosystems suggests that rapid growth …

Ribosome and protein synthesis lie at the core of cell growth and are major consumers of
the cellular budget. Here we review recent progress in the coupling of ribosome synthesis …

Praise for the first edition:… superb, beautifully written and organized work that takes an
engineering approach to systems biology. Alon provides nicely written appendices to …

To respond to fluctuating conditions, microbes typically need to synthesize novel proteins.
As this synthesis relies on sufficient biosynthetic precursors, microbes must devise effective …

For cells to grow faster they must increase their protein production rate. Microorganisms
have traditionally been thought to accomplish this increase by producing more ribosomes to …

It has long been known that bacteria coordinate their physiology with their nutrient
environment, yet our current understanding offers little intuition for how bacteria respond to …

Growth and environmental responses are essential for living organisms to survive and adapt
to constantly changing environments. In order to simulate new conditions and capture …

Failure of modularity remains a significant challenge for assembling synthetic gene circuits
with tested modules as they often do not function as expected. Competition over shared …

Changing nutritional conditions challenge microbes and shape their evolutionary
optimization. Here, we used real-time metabolomics to investigate the role of glycogen in the …

Within a cell, synthetic and native genes compete for expression machinery, influencing
cellular process dynamics through resource couplings. Models that simplify competitive …