[HTML][HTML] Microbial cell factories for the sustainable manufacturing of B vitamins
Highlights•Industrially produced B vitamins are widely used in feed, food, cosmetics and
pharma.•Most B vitamins are produced by chemical synthesis in a non-sustainable …
pharma.•Most B vitamins are produced by chemical synthesis in a non-sustainable …
Shining a light on enzyme promiscuity
SD Copley - Current opinion in structural biology, 2017 - Elsevier
Highlights•Screening efforts have revealed enzymes with over 100 promiscuous
activities.•High-throughput screening for promiscuous activities can be accomplished in …
activities.•High-throughput screening for promiscuous activities can be accomplished in …
Retrosynthetic design of metabolic pathways to chemicals not found in nature
Biology produces a universe of chemicals whose precision and complexity is the envy of
chemists. Over the last 30 years, the expansive field of metabolic engineering has many …
chemists. Over the last 30 years, the expansive field of metabolic engineering has many …
Metabolite–enzyme coevolution: from single enzymes to metabolic pathways and networks
How individual enzymes evolved is relatively well understood. However, individual enzymes
rarely confer a physiological advantage on their own. Judging by its current state, the …
rarely confer a physiological advantage on their own. Judging by its current state, the …
An evolutionary biochemist's perspective on promiscuity
SD Copley - Trends in biochemical sciences, 2015 - cell.com
Evolutionary biochemists define enzyme promiscuity as the ability to catalyze secondary
reactions that are physiologically irrelevant, either because they are too inefficient to affect …
reactions that are physiologically irrelevant, either because they are too inefficient to affect …
Evolution of new functions de novo and from preexisting genes
DI Andersson… - Cold Spring Harbor …, 2015 - cshperspectives.cshlp.org
How the enormous structural and functional diversity of new genes and proteins was
generated (estimated to be 1010–1012 different proteins in all organisms on earth [Choi IG …
generated (estimated to be 1010–1012 different proteins in all organisms on earth [Choi IG …
The emergence and early evolution of biological carbon-fixation
The fixation of into living matter sustains all life on Earth, and embeds the biosphere within
geochemistry. The six known chemical pathways used by extant organisms for this function …
geochemistry. The six known chemical pathways used by extant organisms for this function …
A latent capacity for evolutionary innovation through exaptation in metabolic systems
Some evolutionary innovations may originate non-adaptively as exaptations, or pre-
adaptations, which are by-products of other adaptive traits,,,,. Examples include feathers …
adaptations, which are by-products of other adaptive traits,,,,. Examples include feathers …
Adaptive laboratory evolution recruits the promiscuity of succinate semialdehyde dehydrogenase to repair different metabolic deficiencies
Promiscuous enzymes often serve as the starting point for the evolution of novel functions.
Yet, the extent to which the promiscuity of an individual enzyme can be harnessed several …
Yet, the extent to which the promiscuity of an individual enzyme can be harnessed several …
The limits of enzyme specificity and the evolution of metabolism
A Peracchi - Trends in biochemical sciences, 2018 - cell.com
The substrate specificity of enzymes is bound to be imperfect, because of unavoidable
physicochemical limits. In extant metabolic enzymes, furthermore, such limits are seldom …
physicochemical limits. In extant metabolic enzymes, furthermore, such limits are seldom …