Approaches for bacteriophage genome engineering

M Mahler, AR Costa, SPB van Beljouw… - Trends in …, 2023 - cell.com
In recent years, bacteriophage research has been boosted by a rising interest in using
phage therapy to treat antibiotic-resistant bacterial infections. In addition, there is a desire to …

Genetic engineering of bacteriophages: Key concepts, strategies, and applications

W Hussain, X Yang, M Ullah, H Wang, A Aziz… - Biotechnology …, 2023 - Elsevier
Bacteriophages are the most abundant biological entity in the world and hold a tremendous
amount of unexplored genetic information. Since their discovery, phages have drawn a great …

Bacteriophage and endolysin engineering for biocontrol of food pathogens/pathogens in the food: recent advances and future trends

C Lee, H Kim, S Ryu - CritiCal reviews in Food sCienCe and …, 2023 - Taylor & Francis
Despite advances in modern technologies, various foodborne outbreaks have continuously
threatened the food safety. The overuse of and abuse/misuse of antibiotics have escalated …

Engineering Phages to Fight Multidrug-Resistant Bacteria

H Peng, IA Chen, U Qimron - Chemical Reviews, 2024 - ACS Publications
Facing the global “superbug” crisis due to the emergence and selection for antibiotic
resistance, phages are among the most promising solutions. Fighting multidrug-resistant …

T4 phage display technology for enhanced photodynamic therapy of breast cancer

XL Hou, XT **e, LF Tan, F Zhang, JX Fan… - ACS Materials …, 2023 - ACS Publications
Hypoxia in tumor tissues is the major obstacle to photodynamic therapy (PDT). Herein, a self-
oxygenating nanoplatform T4-Ce6-Cat (T4CCa) is used for improving PDT. T4 acts as a …

Engineering bacteriophages for enhanced host range and efficacy: Insights from bacteriophage-bacteria interactions

HJ Jia, PP Jia, S Yin, LK Bu, G Yang… - Frontiers in Microbiology, 2023 - frontiersin.org
Bacteriophages, the most abundant organisms on earth, have the potential to address the
rise of multidrug-resistant bacteria resulting from the overuse of antibiotics. However, their …

Temporal epigenome modulation enables efficient bacteriophage engineering and functional analysis of phage DNA modifications

N Pozhydaieva, FA Billau, M Wolfram-Schauerte… - PLoS …, 2024 - journals.plos.org
Lytic bacteriophages hold substantial promise in medical and biotechnological applications.
Therefore a comprehensive understanding of phage infection mechanisms is crucial …

T4 bacteriophage nanoparticles engineered through CRISPR provide a versatile platform for rapid development of flu mucosal vaccines

M Li, C Chen, X Wang, P Guo, H Feng, X Zhang… - Antiviral Research, 2023 - Elsevier
Vaccines that trigger mucosal immune responses at the entry portals of pathogens are
highly desired. Here, we showed that antigen-decorated nanoparticle generated through …

Monomeric streptavidin phage display allows efficient immobilization of bacteriophages on magnetic particles for the capture, separation, and detection of bacteria

CM Carmody, SR Nugen - Scientific Reports, 2023 - nature.com
Immobilization of bacteriophages onto solid supports such as magnetic particles has
demonstrated ultralow detection limits as biosensors for the separation and detection of their …

Landscape of new nuclease-containing antiphage systems in Escherichia coli and the counterdefense roles of bacteriophage T4 genome modifications

S Wang, E Sun, Y Liu, B Yin, X Zhang, M Li… - Journal of …, 2023 - Am Soc Microbiol
Many phages, such as T4, protect their genomes against the nucleases of bacterial
restriction-modification (RM) and CRISPR-Cas systems through covalent modification of …