Scanning probe microscopy

K Bian, C Gerber, AJ Heinrich, DJ Müller… - Nature Reviews …, 2021 - nature.com
Scanning probe microscopy (SPM), a key invention in nanoscience, has by now been
extended to a wide spectrum of basic and applied fields. Its application to basic science led …

Challenges and opportunities in cryo-EM single-particle analysis

D Lyumkis - Journal of Biological Chemistry, 2019 - ASBMB
Cryogenic electron microscopy (cryo-EM) enables structure determination of
macromolecular objects and their assemblies. Although the techniques have been …

New tools for automated cryo-EM single-particle analysis in RELION-4.0

D Kimanius, L Dong, G Sharov, T Nakane… - Biochemical …, 2021 - portlandpress.com
We describe new tools for the processing of electron cryo-microscopy (cryo-EM) images in
the fourth major release of the RELION software. In particular, we introduce VDAM, a …

CD147-spike protein is a novel route for SARS-CoV-2 infection to host cells

KE Wang, W Chen, Z Zhang, Y Deng, JQ Lian… - Signal transduction and …, 2020 - nature.com
In face of the everlasting battle toward COVID-19 and the rapid evolution of SARS-CoV-2, no
specific and effective drugs for treating this disease have been reported until today …

CryoDRGN: reconstruction of heterogeneous cryo-EM structures using neural networks

ED Zhong, T Bepler, B Berger, JH Davis - Nature methods, 2021 - nature.com
Cryo-electron microscopy (cryo-EM) single-particle analysis has proven powerful in
determining the structures of rigid macromolecules. However, many imaged protein …

Nuclear pores dilate and constrict in cellulo

CE Zimmerli, M Allegretti, V Rantos, SK Goetz… - Science, 2021 - science.org
INTRODUCTION The nucleus harbors and protects the genetic information in eukaryotes. It
is surrounded by the nuclear envelope, which separates the nucleoplasm from the cytosol …

Structure-based discovery of small molecules that disaggregate Alzheimer's disease tissue derived tau fibrils in vitro

PM Seidler, KA Murray, DR Boyer, P Ge… - Nature …, 2022 - nature.com
Alzheimer's disease (AD) is the consequence of neuronal death and brain atrophy
associated with the aggregation of protein tau into fibrils. Thus disaggregation of tau fibrils …

SARS-CoV-2 structure and replication characterized by in situ cryo-electron tomography

S Klein, M Cortese, SL Winter… - Nature …, 2020 - nature.com
Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), the causative agent of the
COVID19 pandemic, is a highly pathogenic β-coronavirus. As other coronaviruses, SARS …

Cryo-EM structures of SARS-CoV-2 spike without and with ACE2 reveal a pH-dependent switch to mediate endosomal positioning of receptor-binding domains

T Zhou, Y Tsybovsky, J Gorman, M Rapp, G Cerutti… - Cell host & …, 2020 - cell.com
The SARS-CoV-2 spike employs mobile receptor-binding domains (RBDs) to engage the
human ACE2 receptor and to facilitate virus entry, which can occur through low-pH …

Amyloid fibrils in FTLD-TDP are composed of TMEM106B and not TDP-43

YX Jiang, Q Cao, MR Sawaya, R Abskharon, P Ge… - Nature, 2022 - nature.com
Frontotemporal lobar degeneration (FTLD) is the third most common neurodegenerative
condition after Alzheimer's and Parkinson's diseases. FTLD typically presents in 45 to 64 …