Advanced biotechnology: Metabolically engineered cells for the bio‐based production of chemicals and fuels, materials, and health‐care products

J Becker, C Wittmann - Angewandte Chemie International …, 2015 - Wiley Online Library
Corynebacterium glutamicum, Escherichia coli, and Saccharomyces cerevisiae in particular,
have become established as important industrial workhorses in biotechnology. Recent years …

Engineering biosynthetic enzymes for industrial natural product synthesis

S Galanie, D Entwistle, J Lalonde - Natural Product Reports, 2020 - pubs.rsc.org
Covering: 2000 to 2020 Natural products and their derivatives are commercially important
medicines, agrochemicals, flavors, fragrances, and food ingredients. Industrial strategies to …

Inactivation of immobilized trypsin under dissimilar conditions produces trypsin molecules with different structures

A Sanchez, J Cruz, N Rueda, JCS dos Santos… - RSC …, 2016 - pubs.rsc.org
Bovine trypsin has been immobilized on glyoxyl-agarose and two different preparations
have been produced. One was reduced just after immobilization, while the other was left to …

Harnessing ortho-Quinone Methides in Natural Product Biosynthesis and Biocatalysis

TN Purdy, BS Moore, AL Lukowski - Journal of natural products, 2022 - ACS Publications
The implementation of ortho-quinone methide (o-QM) intermediates in complex molecule
assembly represents a remarkably efficient strategy designed by Nature and utilized by …

Advances, opportunities, and challenges in methods for interrogating the structure activity relationships of natural products

CMF Ancajas, AS Oyedele, CM Butt… - Natural Product …, 2024 - pubs.rsc.org
Time span in literature: 1985-early 2024Natural products play a key role in drug discovery,
both as a direct source of drugs and as a starting point for the development of synthetic …

Controllable Synthesis of Hemoglobin–Metal Phosphate Organic–Inorganic Hybrid Nanoflowers and Their Applications in Biocatalysis

J Gao, H Liu, C Tong - Inorganic Chemistry, 2023 - ACS Publications
In recent years, organic–inorganic hybrid nanoflower technology has become an effective
method for enzyme immobilization. Here, seven hierarchical flower-like hemoglobin …

Repurposing modular polyketide synthases and non-ribosomal peptide synthetases for novel chemical biosynthesis

S Hwang, N Lee, S Cho, B Palsson… - Frontiers in molecular …, 2020 - frontiersin.org
In nature, various enzymes govern diverse biochemical reactions through their specific three-
dimensional structures, which have been harnessed to produce many useful bioactive …

Biosynthetic strategies of berberine bridge enzyme-like flavoprotein oxidases toward structural diversification in natural product biosynthesis

G Tjallinks, A Mattevi, MW Fraaije - Biochemistry, 2024 - ACS Publications
Berberine bridge enzyme-like oxidases are often involved in natural product biosynthesis
and are seen as essential enzymes for the generation of intricate pharmacophores. These …

Decoding and reprogramming complex polyketide assembly lines: prospects for synthetic biology

C Hertweck - Trends in biochemical sciences, 2015 - cell.com
Bacterial modular type I polyketide synthases (PKSs) represent giant megasynthases that
produce a vast number of complex polyketides, many of which are pharmaceutically …

Immobilization of lipases on glyoxyl–octyl supports: Improved stability and reactivation strategies

A Suescun, N Rueda, JCS dos Santos, JJ Castillo… - Process …, 2015 - Elsevier
Lipases from Candida rugosa (CRL) and from Candida antarctica (isoform A)(CALA) have
been successfully immobilized on octyl–glyoxyl agarose (OCGLX) beads and compared to …