The potential use of the φC31 integrase system in gene therapy opens up the possibilities of
new treatments for old diseases. φC31 integrase mediates the integration of plasmid DNA …

Site-specific recombinases (SSRs) have been crucial in the development of mammalian
transgenesis. For gene therapy purposes, this approach remains challenging, because, for …

Phage-encoded serine integrases mediate directionally regulated site-specific
recombination between short attP and attB DNA sites without host factor requirements …

Hemophilia B, a hereditary bleeding disorder caused by a deficiency of coagulation factor IX
(FIX), is an excellent candidate for gene therapy. However, to date, success in hemophilia …

Introduction: The φC31 integrase system is a phage-derived system that offers the ability to
integrate plasmid DNA into the chromosomes at a subset of endogenous preferred locations …

Phage integrases are prokaryotic site-specific recombinases that perform precise cut-and-
paste recombination between their short attB and attP recognition sequences. These …

Background Hydrodynamic injection is an effective method for DNA delivery in mouse liver
and is being translated to larger animals for possible clinical use. Similarly, φC31 integrase …

Objective The Streptomyces phage phiC31 integrase offers a sequence-specific method of
transgenesis with a robust long-term gene expression. PhiC31 has been successfully …

PhiC31 integrase has the potential to achieve long-term transgene expression because of
site-specific and unidirectional recombination. In this study, plasmid DNA (pDNA) encoding …

The integrase from phage φC31 of Streptomyces bacteria is an attractive recombinase for
use in generating transgenic organisms and develo** gene and cell therapeutic …