Targeted nucleases have provided researchers with the ability to manipulate virtually any
genomic sequence, enabling the facile creation of isogenic cell lines and animal models for …

The targeted deletion, replacement, integration or inversion of genomic sequences could be
used to study or treat human genetic diseases, but existing methods typically require double …

Conspectus The understanding of gene regulation and the structure and function of the
human genome increased dramatically at the end of the 20th century. Yet the technologies …

We describe the development of 'recCas9', an RNA-programmed small serine recombinase
that functions in mammalian cells. We fused a catalytically inactive dCas9 to the catalytic …

Inspired by the key role of super-helical motifs in molecular self-organization, several
tandem heptad repeat peptides were used as building blocks to form well-ordered …

Protein engineering is used to generate novel protein folds and assemblages, to impart new
properties and functions onto existing proteins, and to enhance our understanding of …

Recent exponential advances in genome sequencing and engineering technologies have
enabled an unprecedented level of interrogation into the impact of DNA variation (genotype) …

The development of new methods for delivering proteins into cells is a central challenge for
advancing both basic research and therapeutic applications. We previously reported that …

An enthalpy-driven ligand is an ideal probe for practical applications because of the
formation of abundant specific bonds between the ligand and target, compared to an entropy …

Over the past two decades, new technologies enabling targeted modification of plant
genomes have been developed. Among these are zinc-finger nucleases (ZFNs) which are …