Factors affecting the expression of recombinant protein and improvement strategies in Chinese hamster ovary cells

ZM Li, ZL Fan, XY Wang, TY Wang - Frontiers in Bioengineering and …, 2022 - frontiersin.org
Recombinant therapeutic proteins (RTPs) are important parts of biopharmaceuticals.
Chinese hamster ovary cells (CHO) have become the main cell hosts for the production of …

Recent advances of molecular toolbox construction expand Pichia pastoris in synthetic biology applications

Z Kang, H Huang, Y Zhang, G Du, J Chen - World Journal of Microbiology …, 2017 - Springer
Pichia pastoris:(reclassified as Komagataella phaffii), a methylotrophic yeast strain has been
widely used for heterologous protein production because of its unique advantages, such as …

In vivo, in vitro and in silico: an open space for the development of microbe‐based applications of synthetic biology

A Danchin - Microbial Biotechnology, 2022 - Wiley Online Library
Living systems are studied using three complementary approaches: living cells, cell‐free
systems and computer‐mediated modelling. Progresses in understanding, allowing …

Analysis of synonymous codon usage of transcriptome database in Rheum palmatum

X Huo, S Liu, Y Li, H Wei, J Gao, Y Yan, G Zhang, M Liu - PeerJ, 2021 - peerj.com
Background Rheum palmatum is an endangered and important medicinal plant in Asian
countries, especially in China. However, there is little knowledge about the codon usage …

Polysome profiling of mAb producing CHO cell lines links translational control of cell proliferation and recombinant mRNA loading onto ribosomes with global and …

CL Godfrey, EJ Mead, O Daramola, S Dunn… - Biotechnology …, 2017 - Wiley Online Library
Abstract mRNA translation is a key process determining growth, proliferation and duration of
a Chinese hamster ovary (CHO) cell culture and influences recombinant protein synthesis …