Highlights•Miniaturization is the key towards microbial single-cell MS.•Smart integration of
cell handling, incubation, and analyte ionization is mandatory.•Volatile cell-compatible …

Cellular heterogeneity is prevalent in all cell populations. Single-cell analysis (SCA) could
unveil the heterogeneity and thus gain unique insights into complex biological processes …

Improving the performance of chemical transformations catalysed by microbial biocatalysts
requires a deep understanding of cellular processes. While the cellular heterogeneity of …

Highlights•Droplet-MS offers selective, high-throughput analysis for low volume
samples.•Applications include drug screening, chemical reaction development, and in vivo …

Synthetic biology relies on engineering cells to have desirable properties, such as the
production of select chemicals. A bottleneck in engineering methods is often the need to …

In this work, we introduce an approach to merge droplet microfluidics with an HPLC/MS
functionality on a single chip to analyze the contents of individual droplets. This is achieved …

Advances in microbiology rely on innovations in technology. Droplet microfluidics, as a
versatile and powerful technique that allows high-throughput generation and manipulation …

Precise manipulation, fast cultivation, and sensitive detection of fungi and bacteria are vital
for behavior monitoring, phenotypic identification, physiology assessment, and molecular …

This study presents the label-free sorting of cyanobacterial cells in droplets with single-cell
sensitivity based on their fluorescence lifetime. We separated living and dead cyanobacteria …

We introduce the coupling of droplet microfluidics and ion mobility spectrometry (IMS) to
address the challenges of label-free and chemical-specific detection of compounds in …