Kinetochores are large protein assemblies that connect chromosomes to microtubules of the
mitotic and meiotic spindles in order to distribute the replicated genome from a mother cell to …

The 3D organization of mammalian chromatin was described more than 30 years ago by
visualizing sites of DNA synthesis at different times during the S phase of the cell cycle …

SMC (structural maintenance of chromosomes) complexes—which include condensin,
cohesin and the SMC5–SMC6 complex—are major components of chromosomes in all …

The accurate and complete replication of genomic DNA is essential for all life. In eukaryotic
cells, the assembly of the multi-enzyme replisomes that perform replication is divided into …

Reflecting its pleiotropic functions, Polo-like kinase 1 (PLK1) localizes to various sub-cellular
structures during mitosis. At kinetochores, PLK1 contributes to microtubule attachments and …

Current temporal studies of DNA replication are either low-resolution or require complex cell
synchronisation and/or sorting procedures. Here we introduce Nanotiming, a single …

Replication of eukaryotic genomes is highly stochastic, making it difficult to determine the
replication dynamics of individual molecules with existing methods. We report a sequencing …

The ring-shaped cohesin complex brings together distant DNA domains to maintain,
express, and segregate the genome. Establishing specific chromosomal linkages depends …

Eukaryotic genomes are replicated from multiple DNA replication origins. We present
complementary deep sequencing approaches to measure origin location and activity in …

DNA replicates once per cell cycle. Interfering with the regulation of DNA replication
initiation generates genome instability through over-replication and has been linked to early …