Tryptophan synthase: a multienzyme complex with an intramolecular tunnel

EW Miles - The Chemical Record, 2001 - Wiley Online Library
Tryptophan synthase is a classic enzyme that channels a metabolic intermediate, indole.
The crystal structure of the tryptophan synthase α2β2 complex from Salmonella typhimurium …

Tryptophan synthase: a mine for enzymologists

S Raboni, S Bettati, A Mozzarelli - Cellular and molecular life sciences, 2009 - Springer
Tryptophan synthase is a pyridoxal 5′-phosphate-dependent α 2 β 2 complex catalyzing
the last two steps of tryptophan biosynthesis in bacteria, plants and fungi. Structural …

Loop Closure and Intersubunit Communication in Tryptophan Synthase,

TR Schneider, E Gerhardt, M Lee, PH Liang… - Biochemistry, 1998 - ACS Publications
Crystal structures of wild-type tryptophan synthase α2β2 complexes from Salmonella
typhimurium were determined to investigate the mechanism of allosteric activation of the α …

Domain architecture of the heme-independent yeast cystathionine β-synthase provides insights into mechanisms of catalysis and regulation

KH Jhee, P McPhie, EW Miles - Biochemistry, 2000 - ACS Publications
Cystathionine β-synthase from yeast (Saccharomyces cerevisiae) provides a model system
for understanding some of the effects of disease-causing mutations in the human enzyme …

Protonation states of the tryptophan synthase internal aldimine active site from solid-state NMR spectroscopy: direct observation of the protonated Schiff base linkage …

BG Caulkins, B Bastin, C Yang… - Journal of the …, 2014 - ACS Publications
The acid–base chemistry that drives catalysis in pyridoxal-5′-phosphate (PLP)-dependent
enzymes has been the subject of intense interest and investigation since the initial …

Pyridoxal 5′-phosphate-dependent enzymes at the crossroads of host–microbe tryptophan metabolism

B Cellini, T Zelante, M Dindo, MM Bellet… - International Journal of …, 2020 - mdpi.com
The chemical processes taking place in humans intersects the myriad of metabolic pathways
occurring in commensal microorganisms that colonize the body to generate a complex …

Exploring and exploiting allostery: Models, evolution, and drug targeting

A Peracchi, A Mozzarelli - Biochimica et Biophysica Acta (BBA)-Proteins …, 2011 - Elsevier
The concept of allostery was elaborated almost 50years ago by Monod and coworkers to
provide a framework for interpreting experimental studies on the regulation of protein …

Cooperative fluctuations and subunit communication in tryptophan synthase

I Bahar, RL Jernigan - Biochemistry, 1999 - ACS Publications
Tryptophan synthase (TRPS), with linearly arrayed subunits αββα, catalyzes the last two
reactions in the biosynthesis of l-tryptophan. The two reactions take place in the respective α …

The enzymology of cystathionine biosynthesis: strategies for the control of substrate and reaction specificity

SM Aitken, JF Kirsch - Archives of biochemistry and biophysics, 2005 - Elsevier
The ability of enzymes to catalyze specific reactions, while excluding others, is central to
cellular metabolism. Control of reaction specificity is of particular importance for enzymes …

Allosteric regulation of substrate channeling: Salmonella typhimurium tryptophan synthase

RK Ghosh, E Hilario, CA Chang, LJ Mueller… - Frontiers in Molecular …, 2022 - frontiersin.org
The regulation of the synthesis of L-tryptophan (L-Trp) in enteric bacteria begins at the level
of gene expression where the cellular concentration of L-Trp tightly controls expression of …