High-throughput Raman flow cytometry and beyond

J Gala de Pablo, M Lindley, K Hiramatsu… - Accounts of Chemical …, 2021 - ACS Publications
Conspectus Flow cytometry is a powerful tool with applications in diverse fields such as
microbiology, immunology, virology, cancer biology, stem cell biology, and metabolic …

Engineered virus-like particles for efficient in vivo delivery of therapeutic proteins

S Banskota, A Raguram, S Suh, SW Du, JR Davis… - Cell, 2022 - cell.com
Methods to deliver gene editing agents in vivo as ribonucleoproteins could offer safety
advantages over nucleic acid delivery approaches. We report the development and …

Engineered virus-like particles for transient delivery of prime editor ribonucleoprotein complexes in vivo

M An, A Raguram, SW Du, S Banskota, JR Davis… - Nature …, 2024 - nature.com
Prime editing enables precise installation of genomic substitutions, insertions and deletions
in living systems. Efficient in vitro and in vivo delivery of prime editing components, however …

[HTML][HTML] From Genesis to Old Age: Exploring the Immune System One Cell at a Time with Flow Cytometry

A Larbi - Biomedicines, 2024 - mdpi.com
The immune system is a highly complex and tightly regulated system that plays a crucial role
in protecting the body against external threats, such as pathogens, and internal …

Quantitative assessment of the physical virus titer and purity by ultrasensitive flow virometry

Q Niu, L Ma, S Zhu, L Li, Q Zheng, J Hou… - Angewandte …, 2021 - Wiley Online Library
Rapid quantification of viruses is vital for basic research on viral diseases as well as
biomedical application of virus‐based products. Here, we report the development of a high …

Optical quantification by nanopores of viruses, extracellular vesicles, and nanoparticles

L Chazot-Franguiadakis, J Eid, M Socol, B Molcrette… - Nano Letters, 2022 - ACS Publications
Nanopores combined with optical approaches can be used to detect viral particles. In this
work, we demonstrate the ability of hydrodynamical driving and optical sensing to identify …

Advances and opportunities in process analytical technologies for viral vector manufacturing

SA Sripada, M Hosseini, S Ramesh, J Wang… - Biotechnology …, 2024 - Elsevier
Viral vectors are an emerging, exciting class of biologics whose application in vaccines,
oncology, and gene therapy has grown exponentially in recent years. Following first …

Viral Membrane-Targeting Amphipathic Helical Peptide-Based Fluorogenic Probes for the Analysis of Infectious Titers of Enveloped Viruses

Y Sato, Y Hatanaka, Y Sato, K Matsumoto… - Analytical …, 2025 - ACS Publications
Enveloped viruses have caused the majority of epidemics and pandemics over the past
decade. Direct sensing of virus particles (virions) holds great potential for the functional …

Flow virometry: recent advancements, best practices, and future frontiers

C Fernandes, AT Persaud, D Chaphekhar… - Journal of …, 2025 - journals.asm.org
The imperative for develo** robust tools to detect, analyze, and characterize viruses has
become increasingly evident as they continue to threaten human health. In this review, we …

Applying Flow Virometry to Study the HIV Envelope Glycoprotein and Differences across HIV Model Systems

J Burnie, C Fernandes, A Patel, AT Persaud… - Viruses, 2024 - mdpi.com
The HIV envelope glycoprotein (Env) is a trimeric protein that facilitates viral binding and
fusion with target cells. As the sole viral protein on the HIV surface, Env is important both for …