Since the advent of induced pluripotent stem cell (iPSC) technology a decade ago,
enormous progress has been made in stem cell biology and regenerative medicine. Human …

The past 10 years have seen great advances in our ability to manipulate cell fate, including
the induction of pluripotency in vitro to generate induced pluripotent stem cells (iPSCs). This …

Understanding how a subset of expressed genes dictates cellular phenotype is a
considerable challenge owing to the large numbers of molecules involved, their …

Organoids capable of forming tissue-like structures have transformed our ability to model
human development and disease. With the notable exception of the human heart, lineage …

The increasing prevalence of diabetes has resulted in a global epidemic. Diabetes is a
major cause of blindness, kidney failure, heart attacks, stroke and amputation of lower limbs …

CRISPR/Cas9 has revolutionized our ability to engineer genomes and conduct genome-
wide screens in human cells,–. Whereas some cell types are amenable to genome …

Human brain development involves complex interactions between different regions,
including long-distance neuronal migration or formation of major axonal tracts. Different …

Human pluripotent stem cell-derived cardiomyocytes (hPSC-CMs) offer a promising cell-
based therapy for myocardial infarction. However, the presence of transitory ventricular …

Genome-wide screening using CRISPR coupled with nuclease Cas9 (CRISPR–Cas9) is a
powerful technology for the systematic evaluation of gene function. Statistically principled …

Background Precise genome editing via homology-directed repair (HDR) after double-
stranded DNA (dsDNA) cleavage facilitates functional genomic research and holds promise …