Performance and application of 16S rRNA gene cycle sequencing for routine identification of bacteria in the clinical microbiology laboratory
DL Church, L Cerutti, A Gürtler, T Griener… - Clinical microbiology …, 2020 - Am Soc Microbiol
This review provides a state-of-the-art description of the performance of Sanger cycle
sequencing of the 16S rRNA gene for routine identification of bacteria in the clinical …
sequencing of the 16S rRNA gene for routine identification of bacteria in the clinical …
[HTML][HTML] Long walk to genomics: History and current approaches to genome sequencing and assembly
Genomes represent the starting point of genetic studies. Since the discovery of DNA
structure, scientists have devoted great efforts to determine their sequence in an exact way …
structure, scientists have devoted great efforts to determine their sequence in an exact way …
Vaccination induces HIV broadly neutralizing antibody precursors in humans
Broadly neutralizing antibodies (bnAbs) can protect against HIV infection but have not been
induced by human vaccination. A key barrier to bnAb induction is vaccine priming of rare …
induced by human vaccination. A key barrier to bnAb induction is vaccine priming of rare …
Transmission of SARS-CoV-2 in free-ranging white-tailed deer in the United States
SARS-CoV-2 is a zoonotic virus with documented bi-directional transmission between
people and animals. Transmission of SARS-CoV-2 from humans to free-ranging white-tailed …
people and animals. Transmission of SARS-CoV-2 from humans to free-ranging white-tailed …
Towards complete and error-free genome assemblies of all vertebrate species
High-quality and complete reference genome assemblies are fundamental for the
application of genomics to biology, disease, and biodiversity conservation. However, such …
application of genomics to biology, disease, and biodiversity conservation. However, such …
Merqury: reference-free quality, completeness, and phasing assessment for genome assemblies
Recent long-read assemblies often exceed the quality and completeness of available
reference genomes, making validation challenging. Here we present Merqury, a novel tool …
reference genomes, making validation challenging. Here we present Merqury, a novel tool …
Sexual-dimorphism in human immune system aging
EJ Márquez, C Chung, R Marches, RJ Rossi… - Nature …, 2020 - nature.com
Differences in immune function and responses contribute to health-and life-span disparities
between sexes. However, the role of sex in immune system aging is not well understood …
between sexes. However, the role of sex in immune system aging is not well understood …
Proposed minimal standards for the use of genome data for the taxonomy of prokaryotes
Advancement of DNA sequencing technology allows the routine use of genome sequences
in the various fields of microbiology. The information held in genome sequences proved to …
in the various fields of microbiology. The information held in genome sequences proved to …
Canu: scalable and accurate long-read assembly via adaptive k-mer weighting and repeat separation
Long-read single-molecule sequencing has revolutionized de novo genome assembly and
enabled the automated reconstruction of reference-quality genomes. However, given the …
enabled the automated reconstruction of reference-quality genomes. However, given the …
Falco: high-speed FastQC emulation for quality control of sequencing data
Quality control is an essential first step in sequencing data analysis, and software tools for
quality control are deeply entrenched in standard pipelines at most sequencing centers …
quality control are deeply entrenched in standard pipelines at most sequencing centers …