Gene Therapy for Sickle Cell Disease: Recent Advances, Clinical Trials, and Future directions

J Ballantine, JF Tisdale - Cytotherapy, 2024 - Elsevier
Abstract Sickle Cell Disease (SCD) is the most common inherited blood disorder worldwide,
impacting millions and imposing severe healthcare challenges, particularly in resource …

Preclinical Efficacy of a Modified Gamma Globin Lentiviral Vector Mediated Gene Therapy in Berkeley Sickle Cell Anemia Mouse and Human Xenograft Models

A Shrestha, DM Pillis, S Felker, M Chi, K Wagner… - … Therapy Methods & …, 2025 - cell.com
We previously showed correction of sickle cell anemia (SCA) in mice utilizing a lentiviral
vector (LV) expressing human γ-globin. Herein, we made a G16D mutation in the γ-globin …

[HTML][HTML] The Optimized γ-Globin Lentiviral Vector GGHI-mB-3D Leads to Nearly Therapeutic HbF Levels In Vitro in CD34+ Cells from Sickle Cell Disease Patients

E Drakopoulou, M Georgomanoli, CW Lederer… - Viruses, 2022 - mdpi.com
We have previously demonstrated that both the original γ-globin lentiviral vector (LV) GGHI
and the optimized GGHI-mB-3D LV, carrying the novel regulatory elements of the 3D HPFH …

Large-scale discovery of potent, compact and lineage specific enhancers for gene therapy vectors

N Psatha, P Sova, G Georgolopoulos, K Paschoudi… - bioRxiv, 2023 - biorxiv.org
Regulation of gene expression during cell development and differentiation is chiefly
orchestrated by distal noncoding regulatory elements that precisely modulate cell selective …

[PDF][PDF] Identification of genome safe harbor loci for human gene therapy based on evolutionary biology and comparative genomics

MA Quezada-Ramírez, S Loncar, MA Campbell… - bioRxiv, 2023 - researchgate.net
Gene transfer into hematopoietic stem and progenitor cells (HSPCs) involving
indiscriminately integrating viral vectors has unpredictable outcomes including potential …

Development of evolutionarily conserved viral integration sites as safe harbors for human gene therapy

MA Quezada-Ramírez, S Loncar, MA Campbell… - bioRxiv, 2023 - biorxiv.org
Gene transfer into CD34+ hematopoietic stem and progenitor cells (HSPCs) involving
integrating viral vectors has unpredictable outcomes including potential adverse events like …