CRISPR screens are a powerful source of biological discovery, enabling the unbiased
interrogation of gene function in a wide range of applications and species. In pooled …

The past decade has witnessed a rapid evolution in identifying more versatile clustered
regularly interspaced short palindromic repeats (CRISPR)/CRISPR-associated protein (Cas) …

CRISPR loss of function screens are powerful tools to interrogate biology but exhibit a
number of biases and artifacts that can confound the results. Here, we introduce Chronos …

Functional genomics approaches can overcome limitations—such as the lack of
identification of robust targets and poor clinical efficacy—that hamper cancer drug …

Abstract The Biological General Repository for Interaction Datasets (BioGRID:
https://thebiogrid. org) is an open access database dedicated to the curation and archival …

The genetic circuits that allow cancer cells to evade destruction by the host immune system
remain poorly understood,–. Here, to identify a phenotypically robust core set of genes and …

Glioblastomas harbor diverse cell populations, including rare glioblastoma stem cells
(GSCs) that drive tumorigenesis. To characterize functional diversity within this population …

Linking variants from genome-wide association studies (GWAS) to underlying mechanisms
of disease remains a challenge,–. For some diseases, a successful strategy has been to …

Although single-gene perturbation screens have revealed a number of new targets,
vulnerabilities specific to frequently altered drivers have not been uncovered. An important …

Genome-wide screening using CRISPR coupled with nuclease Cas9 (CRISPR–Cas9) is a
powerful technology for the systematic evaluation of gene function. Statistically principled …