Mechanism-based design of efficient PET hydrolases

R Wei, G von Haugwitz, L Pfaff, J Mican… - ACS …, 2022 - ACS Publications
Polyethylene terephthalate (PET) is the most widespread synthetic polyester, having been
utilized in textile fibers and packaging materials for beverages and food, contributing …

Multiple substrate binding mode-guided engineering of a thermophilic PET hydrolase

L Pfaff, J Gao, Z Li, A Jäckering, G Weber… - ACS …, 2022 - ACS Publications
Thermophilic polyester hydrolases (PES-H) have recently enabled biocatalytic recycling of
the mass-produced synthetic polyester polyethylene terephthalate (PET), which has found …

Structural insights into (tere) phthalate-ester hydrolysis by a carboxylesterase and its role in promoting PET depolymerization

G von Haugwitz, X Han, L Pfaff, Q Li, H Wei, J Gao… - ACS …, 2022 - ACS Publications
TfCa, a promiscuous carboxylesterase from Thermobifida fusca, was found to hydrolyze
polyethylene terephthalate (PET) degradation intermediates such as bis (2-hydroxyethyl) …

Engineering and evaluation of thermostable IsPETase variants for PET degradation

S Brott, L Pfaff, J Schuricht, JN Schwarz… - Engineering in life …, 2022 - Wiley Online Library
Polyethylene terephthalate (PET) is a mass‐produced petroleum‐based synthetic polymer.
Enzymatic PET degradation using, for example, Ideonella sakaiensis PETase (IsPETase) …

Ancestral Sequence Reconstruction Identifies Structural Changes Underlying the Evolution of Ideonella sakaiensis PETase and Variants with Improved Stability and …

Y Joho, V Vongsouthi, MA Spence, J Ton, C Gomez… - Biochemistry, 2022 - ACS Publications
The improved production, recycling, and removal of plastic waste, such as polyethylene
terephthalate (PET), are pressing environmental and economic issues for society …

[HTML][HTML] Hel** proteins come in from the cold: 5 burning questions about cold-active enzymes

JS Nowak, DE Otzen - BBA advances, 2024 - Elsevier
Enzymes from psychrophilic (cold-loving) organisms have attracted considerable interest
over the past decades for their potential in various low-temperature industrial processes …

Synthesis and characterization of polyethylene terephthalate (PET) precursors and potential degradation products: Toxicity study and application in discovery of novel …

M Djapovic, D Milivojevic, T Ilic-Tomic, M Lješević… - Chemosphere, 2021 - Elsevier
Polyethylene terephthalate (PET) is widely used material and as such became highly
enriched in nature. It is generally considered inert and safe plastic, but due to the recent …

Molecular and biochemical differences of the tandem and cold-adapted PET hydrolases Ple628 and Ple629, isolated from a marine microbial consortium

IE Meyer Cifuentes, P Wu, Y Zhao, W Liu… - … in Bioengineering and …, 2022 - frontiersin.org
Polybutylene adipate terephthalate (PBAT) is a biodegradable alternative to polyethylene
and can be broadly used in various applications. These polymers can be degraded by …

Reaction pathways for the enzymatic degradation of poly (ethylene terephthalate): what characterizes an efficient PET‐hydrolase?

S Schubert, K Schaller, JA Bååth, C Hunt… - …, 2023 - Wiley Online Library
Bioprocessing of polyester waste has emerged as a promising tool in the quest for a cyclic
plastic economy. One key step is the enzymatic breakdown of the polymer, and this entails a …

Exploring new galaxies: Perspectives on the discovery of novel PET-degrading enzymes

J Mican, J Da'san MM, W Liu, G Weber… - Applied Catalysis B …, 2024 - Elsevier
Polyethylene terephthalate (PET) is a widely used polyester due to its beneficial material
properties and low cost. However, PET contributes significantly to the growing problem of …