Mass spectrometry-based protein footprinting for higher-order structure analysis: fundamentals and applications
Proteins adopt different higher-order structures (HOS) to enable their unique biological
functions. Understanding the complexities of protein higher-order structures and dynamics …
functions. Understanding the complexities of protein higher-order structures and dynamics …
Arsenic binding to proteins
Arsenic is a trace element found in the earth, s crust at an average concentration of∼ 5 μg/g
(ppm). Although its relative abundance in the earth, s crust is about 54th, arsenic can …
(ppm). Although its relative abundance in the earth, s crust is about 54th, arsenic can …
Hydroxyl radical-mediated modification of proteins as probes for structural proteomics
“Footprinting” refers to assays that examine ligand binding and conformational changes by
determining the solvent accessibility of the backbone, bases, or side chain structures of …
determining the solvent accessibility of the backbone, bases, or side chain structures of …
Chemical modifications in therapeutic protein aggregates generated under different stress conditions
In this study, we characterized the chemical modifications in the monoclonal antibody (IgG 2)
aggregates generated under various conditions, including mechanical, chemical, and …
aggregates generated under various conditions, including mechanical, chemical, and …
Radiolytic protein footprinting with mass spectrometry to probe the structure of macromolecular complexes
Structural proteomics approaches using mass spectrometry are increasingly used in biology
to examine the composition and structure of macromolecules. Hydroxyl radical–mediated …
to examine the composition and structure of macromolecules. Hydroxyl radical–mediated …
The Amyloid-β Peptide of Alzheimer's Disease Binds CuI in a Linear Bis-His Coordination Environment: Insight into a Possible Neuroprotective Mechanism for the …
Oxidative stress has been suggested to contribute to neuronal apoptosis associated with
Alzheimer's disease (AD). Copper may participate in oxidative stress through redox-cycling …
Alzheimer's disease (AD). Copper may participate in oxidative stress through redox-cycling …
Protein structure and dynamics studied by mass spectrometry: H/D exchange, hydroxyl radical labeling, and related approaches
Mass spectrometry (MS) plays a central role in studies on protein structure and dynamics.
This review highlights some of the recent developments in this area, with focus on …
This review highlights some of the recent developments in this area, with focus on …
Using mass spectrometry‐based methods to understand amyloid formation and inhibition of alpha‐synuclein and amyloid beta
Amyloid fibrils, insoluble β‐sheets structures that arise from protein misfolding, are
associated with several neurodegenerative disorders. Many small molecules have been …
associated with several neurodegenerative disorders. Many small molecules have been …
A comparison of methionine, histidine and cysteine in copper(i)-binding peptides reveals differences relevant to copper uptake by organisms in diverse environments
The N-terminal, extracellular regions of eukaryotic high affinity copper transport (Ctr)
proteins vary in composition of the Cu (i) binding amino acids: methionine, histidine, and …
proteins vary in composition of the Cu (i) binding amino acids: methionine, histidine, and …
Structural proteomics of macromolecular assemblies using oxidative footprinting and mass spectrometry
Understanding the composition, structure and dynamics of macromolecules and their
assemblies is at the forefront of biological science today. Hydroxyl-radical-mediated protein …
assemblies is at the forefront of biological science today. Hydroxyl-radical-mediated protein …