Many clustered regularly interspaced short palindromic repeats (CRISPR)-CRISPR-
associated protein 9 (Cas9)-based genome editing technologies take advantage of Cas …

In the mitochondrial outer membrane, α-helical transmembrane proteins play critical roles in
cytoplasmic-mitochondrial communication. Using genome-wide CRISPR screens, we …

Cells repair DNA double-strand breaks (DSBs) through a complex set of pathways critical for
maintaining genomic integrity. To systematically map these pathways, we developed a high …

Gene therapy is a fast develo** field of medicine with hundreds of ongoing early-stage
clinical trials and numerous preclinical studies. Genome editing (GE) now is an increasingly …

The efficiency of targeted knock-in for cell therapeutic applications is generally low, and the
scale is limited. In this study, we developed CLASH, a system that enables high-efficiency …

Ribosome-associated quality control (RQC) pathways protect cells from toxicity caused by
incomplete protein products resulting from translation of damaged or problematic mRNAs …

CRISPR/Cas9 genome-editing tools have tremendously boosted our capability of
manipulating the eukaryotic genomes in biomedical research and innovative …

Abstract β-thalassemias (β-thal) are a group of blood disorders caused by mutations in the β-
globin gene (HBB) cluster. β-globin associates with α-globin to form adult hemoglobin (HbA …

Defining the subcellular distribution of all human proteins and their remodeling across
cellular states remains a central goal in cell biology. Here, we present a high-resolution …

Bacterial transposons propagate through either non-replicative (cut-and-paste) or replicative
(copy-and-paste) pathways, depending on how the mobile element is excised from its donor …