Extracellular vesicle analysis

A Hendrix, L Lippens, C Pinheiro, C Théry… - Nature Reviews …, 2023 - nature.com
Cells release small, phospholipid membrane-enclosed particles, collectively referred to as
extracellular vesicles (EVs), into their surroundings to enable intercellular communication …

Proteomics Profiling of Extracellular Vesicle for Identification of Potential Biomarkers in Alzheimer's Disease: A Comprehensive Review

JJ Pei, CP Palanisamy, S Jayaraman… - Ageing Research …, 2024 - Elsevier
The intricate origins and diverse symptoms of Alzheimer's disease (AD) pose significant
challenges for both diagnosis and treatment. Exosomes and microvesicles, which carry …

Cell culture‐derived extracellular vesicles: Considerations for reporting cell culturing parameters

F Shekari, FJ Alibhai, H Baharvand… - Journal of …, 2023 - Wiley Online Library
Cell culture‐conditioned medium (CCM) is a valuable source of extracellular vesicles (EVs)
for basic scientific, therapeutic and diagnostic applications. Cell culturing parameters affect …

Identification and validation of extracellular vesicle reference genes for the normalization of RT‐qPCR data

C Pinheiro, N Guilbert, L Lippens… - Journal of …, 2024 - Wiley Online Library
Extracellular vesicles (EVs) contain a plethora of biomolecules, including nucleic acids, with
diverse diagnostic and therapeutic application potential. Although reverse transcription …

Uncovering the gray zone: map** the global landscape of direct-to-consumer businesses offering interventions based on secretomes, extracellular vesicles, and …

A Asadpour, BH Yahaya, K Bicknell, GS Cottrell… - Stem Cell Research & …, 2023 - Springer
Background The last decade has seen a significant increase in media attention, industrial
growth, and patient interest in stem cell-based interventions. This led to a rise in direct-to …

Hyaluronic acid: An overlooked extracellular vesicle contaminant

JP Goncalves, RE Ghebosu, XNS Tan… - Journal of …, 2023 - Wiley Online Library
The variable presence of contaminants in extracellular vesicle (EV) samples is one of the
major contributors to a lack of inter‐study reproducibility in the field. Well‐known …

Bioprinting salivary gland models and their regenerative applications

J Klangprapan, GR Souza, JN Ferreira - BDJ open, 2024 - nature.com
Objective Salivary gland (SG) hypofunction is a common clinical condition arising from
radiotherapy to suppress head and neck cancers. The radiation often destroys the SG …

Exosomes, microvesicles, and other extracellular vesicles—a Keystone Symposia report

J Cable, KW Witwer, RJ Coffey… - Annals of the New …, 2023 - Wiley Online Library
Extracellular vesicles (EVs) are small, lipid‐bilayer‐bound particles released by cells that
can contain important bioactive molecules, including lipids, RNAs, and proteins. Once …

Simultaneous Protein and RNA Analysis in Single Extracellular Vesicles, Including Viruses

Z Troyer, O Gololobova, A Koppula, Z Liao, F Horns… - ACS …, 2024 - ACS Publications
The individual detection of human immunodeficiency virus (HIV) virions and resolution from
extracellular vesicles (EVs) during analysis is a difficult challenge. Infectious enveloped …

Heat inactivation of foetal bovine serum performed after EV‐depletion influences the proteome of cell‐derived extracellular vesicles

O Urzì, M Bergqvist, C Lässer… - Journal of …, 2024 - Wiley Online Library
The release of extracellular vesicles (EVs) in cell cultures as well as their molecular cargo
can be influenced by cell culture conditions such as the presence of foetal bovine serum …