Engineering the next generation of cell-based therapeutics
Cell-based therapeutics are an emerging modality with the potential to treat many currently
intractable diseases through uniquely powerful modes of action. Despite notable recent …
intractable diseases through uniquely powerful modes of action. Despite notable recent …
Engineering living therapeutics with synthetic biology
The steadfast advance of the synthetic biology field has enabled scientists to use genetically
engineered cells, instead of small molecules or biologics, as the basis for the development …
engineered cells, instead of small molecules or biologics, as the basis for the development …
Immuno-oncology: emerging targets and combination therapies
HT Marshall, MBA Djamgoz - Frontiers in oncology, 2018 - frontiersin.org
Host immunity recognizes and eliminates most early tumor cells, yet immunological
checkpoints, exemplified by CTLA-4, PD-1, and PD-L1, pose a significant obstacle to …
checkpoints, exemplified by CTLA-4, PD-1, and PD-L1, pose a significant obstacle to …
Systems and synthetic microRNA biology: from biogenesis to disease pathogenesis
H Matsuyama, HI Suzuki - International journal of molecular sciences, 2019 - mdpi.com
MicroRNAs (miRNAs) are approximately 22-nucleotide-long, small non-coding RNAs that
post-transcriptionally regulate gene expression. The biogenesis of miRNAs involves multiple …
post-transcriptionally regulate gene expression. The biogenesis of miRNAs involves multiple …
Designing cell function: assembly of synthetic gene circuits for cell biology applications
M **e, M Fussenegger - Nature Reviews Molecular Cell Biology, 2018 - nature.com
Synthetic biology is the discipline of engineering application-driven biological functionalities
that were not evolved by nature. Early breakthroughs of cell engineering, which were based …
that were not evolved by nature. Early breakthroughs of cell engineering, which were based …
Synthetic introns enable splicing factor mutation-dependent targeting of cancer cells
Many cancers carry recurrent, change-of-function mutations affecting RNA splicing factors.
Here, we describe a method to harness this abnormal splicing activity to drive splicing factor …
Here, we describe a method to harness this abnormal splicing activity to drive splicing factor …
A pan-cancer transcriptome analysis reveals pervasive regulation through alternative promoters
Most human protein-coding genes are regulated by multiple, distinct promoters, suggesting
that the choice of promoter is as important as its level of transcriptional activity. However …
that the choice of promoter is as important as its level of transcriptional activity. However …
Engineered dCas9 with reduced toxicity in bacteria: implications for genetic circuit design
Large synthetic genetic circuits require the simultaneous expression of many regulators.
Deactivated Cas9 (dCas9) can serve as a repressor by having a small guide RNA (sgRNA) …
Deactivated Cas9 (dCas9) can serve as a repressor by having a small guide RNA (sgRNA) …
Nanotechnology‐Assisted RNA Delivery: From Nucleic Acid Therapeutics to COVID‐19 Vaccines
In recent years, the main quest of science has been the pioneering of the groundbreaking
biomedical strategies needed for achieving a personalized medicine. Ribonucleic acids …
biomedical strategies needed for achieving a personalized medicine. Ribonucleic acids …
N 1-Methylpseudouridine substitution enhances the performance of synthetic mRNA switches in cells
Synthetic messenger RNA (mRNA) tools often use pseudouridine and 5-methyl cytidine as
substitutions for uridine and cytidine to avoid the immune response and cytotoxicity induced …
substitutions for uridine and cytidine to avoid the immune response and cytotoxicity induced …