Single-cell multi-omics technologies and methods characterize cell states and activities by
simultaneously integrating various single-modality omics methods that profile the …

In the last decade, single-cell RNA sequencing routinely performed on large numbers of
single cells has greatly advanced our understanding of the underlying heterogeneity of …

High-throughput single-cell RNA sequencing has transformed our understanding of complex
cell populations, but it does not provide phenotypic information such as cell-surface protein …

Advances in multiplexed imaging technologies have drastically improved our ability to
characterize healthy and diseased tissues at the single-cell level. Co-detection by indexing …

Advances in single-cell isolation and barcoding technologies offer unprecedented
opportunities to profile DNA, mRNA, and proteins at a single-cell resolution. Recently, bulk …

Genetic screens help infer gene function in mammalian cells, but it has remained difficult to
assay complex phenotypes—such as transcriptional profiles—at scale. Here, we develop …

The recent advent of methods for high-throughput single-cell molecular profiling has
catalyzed a growing sense in the scientific community that the time is ripe to complete the …

INTRODUCTION Single-cell RNA sequencing has demonstrated that both stable cell types
and transient cell states can be discovered and defined by transcriptomes. In situ …

These guidelines are a consensus work of a considerable number of members of the
immunology and flow cytometry community. They provide the theory and key practical …

Technology development in biological research often aims to either increase the number of
cellular features that can be surveyed simultaneously or enhance the resolution at which …