Application of different types of CRISPR/Cas-based systems in bacteria
Z Liu, H Dong, Y Cui, L Cong, D Zhang - Microbial cell factories, 2020 - Springer
As important genome editing tools, CRISPR/Cas systems, especially those based on type II
Cas9 and type V Cas12a, are widely used in genetic and metabolic engineering of bacteria …
Cas9 and type V Cas12a, are widely used in genetic and metabolic engineering of bacteria …
Escherichia coli as a host for metabolic engineering
Over the past century, Escherichia coli has become one of the best studied organisms on
earth. Features such as genetic tractability, favorable growth conditions, well characterized …
earth. Features such as genetic tractability, favorable growth conditions, well characterized …
Editing of the Bacillus subtilis genome by the CRISPR-Cas9 system
J Altenbuchner - Applied and environmental microbiology, 2016 - Am Soc Microbiol
The clustered regularly interspaced short palindromic repeat (CRISPR)-associated (Cas)
systems are adaptive immune systems of bacteria. A type II CRISPR-Cas9 system from …
systems are adaptive immune systems of bacteria. A type II CRISPR-Cas9 system from …
A versatile genetic engineering toolkit for E. coli based on CRISPR-prime editing
CRISPR base editing is a powerful method to engineer bacterial genomes. However, it
restricts editing to single-nucleotide substitutions. Here, to address this challenge, we adapt …
restricts editing to single-nucleotide substitutions. Here, to address this challenge, we adapt …
Consequences of Cas9 cleavage in the chromosome of Escherichia coli
The RNA-guided Cas9 nuclease from CRISPR-Cas systems has emerged as a powerful
biotechnological tool. The specificity of Cas9 can be reprogrammed to cleave desired …
biotechnological tool. The specificity of Cas9 can be reprogrammed to cleave desired …
Advances in industrial biotechnology using CRISPR-Cas systems
PD Donohoue, R Barrangou, AP May - Trends in biotechnology, 2018 - cell.com
The term 'clustered regularly interspaced short palindromic repeats'(CRISPR) has recently
become synonymous with the genome-editing revolution. The RNA-guided endonuclease …
become synonymous with the genome-editing revolution. The RNA-guided endonuclease …
[HTML][HTML] Automating the design-build-test-learn cycle towards next-generation bacterial cell factories
Automation is playing an increasingly significant role in synthetic biology. Groundbreaking
technologies, developed over the past 20 years, have enormously accelerated the …
technologies, developed over the past 20 years, have enormously accelerated the …
Deaminase-mediated multiplex genome editing in Escherichia coli
S Banno, K Nishida, T Arazoe, H Mitsunobu… - Nature …, 2018 - nature.com
In eukaryotes, the CRISPR–Cas9 system has now been widely used as a revolutionary
genome engineering tool,. However, in prokaryotes, the use of nuclease-mediated genome …
genome engineering tool,. However, in prokaryotes, the use of nuclease-mediated genome …
The no-SCAR (Scarless Cas9 Assisted Recombineering) system for genome editing in Escherichia coli
CR Reisch, KLJ Prather - Scientific reports, 2015 - nature.com
Genome engineering methods in E. coli allow for easy to perform manipulations of the
chromosome in vivo with the assistance of the λ-Red recombinase system. These methods …
chromosome in vivo with the assistance of the λ-Red recombinase system. These methods …
CRISPR-Cas9 for the genome engineering of cyanobacteria and succinate production
H Li, CR Shen, CH Huang, LY Sung, MY Wu… - Metabolic …, 2016 - Elsevier
Cyanobacteria hold promise as a cell factory for producing biofuels and bio-derived
chemicals, but genome engineering of cyanobacteria such as Synechococcus elongatus …
chemicals, but genome engineering of cyanobacteria such as Synechococcus elongatus …