BACKGROUND In a growth phase occurring during most of folliculogenesis, the oocyte
produces and accumulates molecules and organelles that are fundamental for the …

Mammalian oocytes go through a long and complex developmental process while acquiring
the competencies that are required for fertilization and embryogenesis. Recent advances in …

With the advent of transgenic technology, which allows the identification of specific gene
activities in develo** mammalian organisms, the house mouse has once again taken a …

BACKGROUND Cryopreservation has been extensively used in assisted reproductive
technology, agriculture and conservation programmes for endangered species. The …

The effect on the microtubule system of human oocytes of cooling to room temperature for
either 10 or 30 minutes has been investigated. Changes in spindle organization were found …

BACKGROUND ICSI is currently the most commonly used assisted reproductive technology,
accounting for 70–80% of the cycles performed. This extensive use, even excessive, is partly …

The first edition of the Textbook of Assisted Reproductive Techniques was published in
2001. As the textbook now enters its sixth edition, some 45 years since the birth of Louise …

Mature mammalian oocytes are poised for completing meiosis II (MII) on fertilization by
positioning the spindle close to an actomyosin-rich cortical cap,,. Here, we show that the …

Female meiosis in higher organisms consists of highly asymmetric divisions, which retain
most maternal stores in the oocyte for embryo development. Asymmetric partitioning of the …

Successful reproduction in mammals requires a competent egg, which is formed during
meiosis through two assymetrical cell divisions. Here, we show that a recently identified …