The primary function of the eye lens is to focus light on the retina. The major proteins in the
lens—α, β, and γ-crystallins—are constantly subjected to age-related changes such as …

Biological research has focused in the past on model organisms and most of the functional
genomics studies in the field of plant sciences are still performed on model species or …

ProLuCID, a new algorithm for peptide identification using tandem mass spectrometry and
protein sequence databases has been developed. This algorithm uses a three tier scoring …

Reliable identification of posttranslational modifications is key to understanding various
cellular regulatory processes. We describe a tool, InsPecT, to identify posttranslational …

A key problem in computational proteomics is distinguishing between correct and false
peptide identifications. We argue that evaluating the error rates of peptide identifications is …

The subject of this tutorial is protein identification and characterisation by database
searching of MS/MS Data. Peptide Mass Fingerprinting is excluded because it is covered in …

Tandem mass spectrometry (MS/MS) has been routinely used to identify peptides from a
protein sequence database. To identify post-translationally modified peptides, most existing …

We have employed recently developed blind modification search techniques to generate the
most comprehensive map of post-translational modifications (PTMs) in human lens …

In proteomics, de novo sequencing is the process of deriving peptide sequences from
tandem mass spectra without the assistance of a sequence database. Such analyses have …

For the identification of novel proteins using MS/MS, de novo sequencing software computes
one or several possible amino acid sequences (called sequence tags) for each MS/MS …