The green fluorescent protein
RY Tsien - Annual review of biochemistry, 1998 - annualreviews.org
In just three years, the green fluorescent protein (GFP) from the jellyfish Aequorea victoria
has vaulted from obscurity to become one of the most widely studied and exploited proteins …
has vaulted from obscurity to become one of the most widely studied and exploited proteins …
[HTML][HTML] Mitochondria, calcium and cell death: a deadly triad in neurodegeneration
Mitochondrial Ca2+ accumulation is a tightly controlled process, in turn regulating functions
as diverse as aerobic metabolism and induction of cell death. The link between Ca2+ (dys) …
as diverse as aerobic metabolism and induction of cell death. The link between Ca2+ (dys) …
The molecular structure of green fluorescent protein
The crystal structure of recombinant wild-type green fluorescent protein (GFP) has been
solved to a resolution of 1.9 Å by multiwavelength anomalous dispersion phasing methods …
solved to a resolution of 1.9 Å by multiwavelength anomalous dispersion phasing methods …
Green fluorescent protein as a noninvasive intracellular pH indicator
M Kneen, J Farinas, Y Li, AS Verkman - Biophysical journal, 1998 - cell.com
It was found that the absorbance and fluorescence of green fluorescent protein (GFP)
mutants are strongly pH dependent in aqueous solutions and intracellular compartments in …
mutants are strongly pH dependent in aqueous solutions and intracellular compartments in …
Photobleaching recovery and anisotropy decay of green fluorescent protein GFP-S65T in solution and cells: cytoplasmic viscosity probed by green fluorescent protein …
The green fluorescent protein (GFP) was used as a noninvasive probe to quantify the
rheological properties of cell cytoplasm. GFP mutant S65T was purified from recombinant …
rheological properties of cell cytoplasm. GFP mutant S65T was purified from recombinant …
Reduced Loading of Intracellular Ca2+ Stores and Downregulation of Capacitative Ca2+Influx in Bcl-2–Overexpressing Cells
P Pinton, D Ferrari, P Magalhães… - The Journal of cell …, 2000 - rupress.org
The mechanism of action of the oncogene bcl-2, a key regulator of the apoptotic process, is
still debated. We have employed organelle-targeted chimeras of the Ca2+-sensitive …
still debated. We have employed organelle-targeted chimeras of the Ca2+-sensitive …
Green Fluorescent Protein Functions as a Reporter for Protein Localization in Escherichia coli
BJ Feilmeier, G Iseminger, D Schroeder… - Journal of …, 2000 - Am Soc Microbiol
The use of green fluorescent protein (GFP) as a reporter for protein localization in
Escherichia coli was explored by creating gene fusions between malE (encoding maltose …
Escherichia coli was explored by creating gene fusions between malE (encoding maltose …
Diffusion of green fluorescent protein in the aqueous-phase lumen of endoplasmic reticulum
The endoplasmic reticulum (ER) is the major compartment for the processing and quality
control of newly synthesized proteins. Green fluorescent protein (GFP) was used as a …
control of newly synthesized proteins. Green fluorescent protein (GFP) was used as a …
Mechanism and cellular applications of a green fluorescent protein-based halide sensor
S Jayaraman, P Haggie, RM Wachter… - Journal of Biological …, 2000 - ASBMB
We report the application of a targetable green fluorescent protein-based cellular halide
indicator. Fluorescence titrations of the purified recombinant yellow fluorescent protein YFP …
indicator. Fluorescence titrations of the purified recombinant yellow fluorescent protein YFP …
Dynamics of the nuclear lamina as monitored by GFP-tagged A-type lamins
JLV Broers, BM Machiels, GJJM van Eys… - Journal of cell …, 1999 - journals.biologists.com
The behavior of chimeric proteins consisting of A-type lamins and green fluorescent protein
(GFP) was studied to investigate the localization and dynamics of nuclear lamins in living …
(GFP) was studied to investigate the localization and dynamics of nuclear lamins in living …