Mutations in the SARS-CoV-2 RNA-dependent RNA polymerase confer resistance to remdesivir by distinct mechanisms
LJ Stevens, AJ Pruijssers, HW Lee… - Science translational …, 2022 - science.org
The nucleoside analog remdesivir (RDV) is a Food and Drug Administration–approved
antiviral for treatment of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) …
antiviral for treatment of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) …
SNP-sites: rapid efficient extraction of SNPs from multi-FASTA alignments
Rapidly decreasing genome sequencing costs have led to a proportionate increase in the
number of samples used in prokaryotic population studies. Extracting single nucleotide …
number of samples used in prokaryotic population studies. Extracting single nucleotide …
DART-seq: an antibody-free method for global m6A detection
KD Meyer - Nature methods, 2019 - nature.com
Abstract N 6-methyladenosine (m6A) is a widespread RNA modification that influences
nearly every aspect of the messenger RNA lifecycle. Our understanding of m6A has been …
nearly every aspect of the messenger RNA lifecycle. Our understanding of m6A has been …
Reprogramming roadmap reveals route to human induced trophoblast stem cells
The reprogramming of human somatic cells to primed or naive induced pluripotent stem
cells recapitulates the stages of early embryonic development,,,,–. The molecular …
cells recapitulates the stages of early embryonic development,,,,–. The molecular …
Quantitative profiling of N6-methyladenosine at single-base resolution in stem-differentiating xylem of Populus trichocarpa using Nanopore direct RNA sequencing
Y Gao, X Liu, B Wu, H Wang, F **, MV Kohnen… - Genome biology, 2021 - Springer
There are no comprehensive methods to identify N 6-methyladenosine (m 6 A) at single-
base resolution for every single transcript, which is necessary for the estimation of m 6 A …
base resolution for every single transcript, which is necessary for the estimation of m 6 A …
Dissecting key regulators of transcriptome kinetics through scalable single-cell RNA profiling of pooled CRISPR screens
We present a combinatorial indexing method, PerturbSci-Kinetics, for capturing whole
transcriptomes, nascent transcriptomes and single guide RNA (sgRNA) identities across …
transcriptomes, nascent transcriptomes and single guide RNA (sgRNA) identities across …
Improving nanopore read accuracy with the R2C2 method enables the sequencing of highly multiplexed full-length single-cell cDNA
R Volden, T Palmer, A Byrne, C Cole… - Proceedings of the …, 2018 - National Acad Sciences
High-throughput short-read sequencing has revolutionized how transcriptomes are
quantified and annotated. However, while Illumina short-read sequencers can be used to …
quantified and annotated. However, while Illumina short-read sequencers can be used to …
Complete vertebrate mitogenomes reveal widespread repeats and gene duplications
Background Modern sequencing technologies should make the assembly of the relatively
small mitochondrial genomes an easy undertaking. However, few tools exist that address …
small mitochondrial genomes an easy undertaking. However, few tools exist that address …
Sci-fate characterizes the dynamics of gene expression in single cells
Gene expression programs change over time, differentiation and development, and in
response to stimuli. However, nearly all techniques for profiling gene expression in single …
response to stimuli. However, nearly all techniques for profiling gene expression in single …
Reconstruction of the human amylase locus reveals ancient duplications seeding modern-day variation
Previous studies suggested that the copy number of the human salivary amylase gene,
AMY1, correlates with starch-rich diets. However, evolutionary analyses are hampered by …
AMY1, correlates with starch-rich diets. However, evolutionary analyses are hampered by …