Toward dynamic structural biology: Two decades of single-molecule Förster resonance energy transfer
BACKGROUND Biomolecular mechanisms are typically inferred from static structural
“snapshots” obtained by x-ray crystallography, nuclear magnetic resonance (NMR) …
“snapshots” obtained by x-ray crystallography, nuclear magnetic resonance (NMR) …
Conformational dynamics of intrinsically disordered proteins regulate biomolecular condensate chemistry
Motions in biomolecules are critical for biochemical reactions. In cells, many biochemical
reactions are executed inside of biomolecular condensates formed by ultradynamic …
reactions are executed inside of biomolecular condensates formed by ultradynamic …
[HTML][HTML] The SARS-CoV-2 nucleocapsid protein is dynamic, disordered, and phase separates with RNA
Abstract The SARS-CoV-2 nucleocapsid (N) protein is an abundant RNA-binding protein
critical for viral genome packaging, yet the molecular details that underlie this process are …
critical for viral genome packaging, yet the molecular details that underlie this process are …
Single-molecule FRET spectroscopy and the polymer physics of unfolded and intrinsically disordered proteins
The properties of unfolded proteins have long been of interest because of their importance
to the protein folding process. Recently, the surprising prevalence of unstructured regions or …
to the protein folding process. Recently, the surprising prevalence of unstructured regions or …
The disordered N-terminal tail of SARS-CoV-2 Nucleocapsid protein forms a dynamic complex with RNA
Abstract The SARS-CoV-2 Nucleocapsid (N) protein is responsible for condensation of the
viral genome. Characterizing the mechanisms controlling nucleic acid binding is a key step …
viral genome. Characterizing the mechanisms controlling nucleic acid binding is a key step …
Single-molecule spectroscopy of protein folding dynamics—expanding scope and timescales
Single-molecule spectroscopy has developed into an important method for probing protein
structure and dynamics, especially in structurally heterogeneous systems. A broad range of …
structure and dynamics, especially in structurally heterogeneous systems. A broad range of …
Quantifying internal friction in unfolded and intrinsically disordered proteins with single-molecule spectroscopy
Internal friction, which reflects the “roughness” of the energy landscape, plays an important
role for proteins by modulating the dynamics of their folding and other conformational …
role for proteins by modulating the dynamics of their folding and other conformational …
Dual-wavelength metalens enables Epi-fluorescence detection from single molecules
Single molecule fluorescence spectroscopy is at the heart of molecular biophysics research
and the most sensitive biosensing assays. The growing demand for precision medicine and …
and the most sensitive biosensing assays. The growing demand for precision medicine and …
FRET-based dynamic structural biology: Challenges, perspectives and an appeal for open-science practices
Single-molecule FRET (smFRET) has become a mainstream technique for studying
biomolecular structural dynamics. The rapid and wide adoption of smFRET experiments by …
biomolecular structural dynamics. The rapid and wide adoption of smFRET experiments by …
Coordinate-dependent diffusion in protein folding
Diffusion on a low-dimensional free-energy surface is a remarkably successful model for the
folding dynamics of small single-domain proteins. Complicating the interpretation of both …
folding dynamics of small single-domain proteins. Complicating the interpretation of both …