Sequence assembly demystified
Advances in sequencing technologies and increased access to sequencing services have
led to renewed interest in sequence and genome assembly. Concurrently, new applications …
led to renewed interest in sequence and genome assembly. Concurrently, new applications …
Upcoming progress of transcriptomics studies on plants: An overview
P Tyagi, D Singh, S Mathur, A Singh… - Frontiers in Plant …, 2022 - frontiersin.org
Transcriptome sequencing or RNA-Sequencing is a high-resolution, sensitive and high-
throughput next-generation sequencing (NGS) approach used to study non-model plants …
throughput next-generation sequencing (NGS) approach used to study non-model plants …
Personalized RNA mutanome vaccines mobilize poly-specific therapeutic immunity against cancer
U Sahin, E Derhovanessian, M Miller, BP Kloke… - Nature, 2017 - nature.com
T cells directed against mutant neo-epitopes drive cancer immunity. However, spontaneous
immune recognition of mutations is inefficient. We recently introduced the concept of …
immune recognition of mutations is inefficient. We recently introduced the concept of …
StringTie enables improved reconstruction of a transcriptome from RNA-seq reads
Methods used to sequence the transcriptome often produce more than 200 million short
sequences. We introduce StringTie, a computational method that applies a network flow …
sequences. We introduce StringTie, a computational method that applies a network flow …
De novo transcript sequence reconstruction from RNA-seq using the Trinity platform for reference generation and analysis
De novo assembly of RNA-seq data enables researchers to study transcriptomes without the
need for a genome sequence; this approach can be usefully applied, for instance, in …
need for a genome sequence; this approach can be usefully applied, for instance, in …
Corset: enabling differential gene expression analysis for de novoassembled transcriptomes
Next generation sequencing has made it possible to perform differential gene expression
studies in non-model organisms. For these studies, the need for a reference genome is …
studies in non-model organisms. For these studies, the need for a reference genome is …
De novo transcriptome assembly: A comprehensive cross-species comparison of short-read RNA-Seq assemblers
Background In recent years, massively parallel complementary DNA sequencing (RNA
sequencing [RNA-Seq]) has emerged as a fast, cost-effective, and robust technology to …
sequencing [RNA-Seq]) has emerged as a fast, cost-effective, and robust technology to …
A comparative study of techniques for differential expression analysis on RNA-Seq data
Recent advances in next-generation sequencing technology allow high-throughput cDNA
sequencing (RNA-Seq) to be widely applied in transcriptomic studies, in particular for …
sequencing (RNA-Seq) to be widely applied in transcriptomic studies, in particular for …
De novo assembly of bacterial transcriptomes from RNA-seq data
B Tjaden - Genome biology, 2015 - Springer
Transcriptome assays are increasingly being performed by high-throughput RNA
sequencing (RNA-seq). For organisms whose genomes have not been sequenced and …
sequencing (RNA-seq). For organisms whose genomes have not been sequenced and …
A comprehensive comparison of RNA-Seq-based transcriptome analysis from reads to differential gene expression and cross-comparison with microarrays: a case …
RNA-seq, has recently become an attractive method of choice in the studies of
transcriptomes, promising several advantages compared with microarrays. In this study, we …
transcriptomes, promising several advantages compared with microarrays. In this study, we …