Immobilized Iron (III) metal affinity chromatography for the separation of phosphorylated macromolecules: ligands and applications
LD Holmes, MR Schiller - Journal of liquid chromatography & …, 1997 - Taylor & Francis
This review highlights the advances in Fe (III)-Immobilized Metal Affinity Chromatography
[Fe (III)-IMAC] for the separation and characterization of phosphorylated biomolecules. Fe …
[Fe (III)-IMAC] for the separation and characterization of phosphorylated biomolecules. Fe …
Conditions Promoting Metal‐Catalyzed Oxidations during Immobilized Cu—Iminodiacetic Acid Metal Affinity Chromatography
R Krishnamurthy, RD Madurawe, KD Bush… - Biotechnology …, 1995 - Wiley Online Library
Many solutions that contain oxygen and/or hydrogen peroxide, transition metals, and
reductants undergo metal‐catalyzed oxidation (MCO) reactions. These reactions produce …
reductants undergo metal‐catalyzed oxidation (MCO) reactions. These reactions produce …
Characterization of metal affinity of green fluorescent protein and its purification through salt promoted, immobilized metal affinity chromatography
Immobilized metal affinity chromatography (IMAC) was investigated as a method of recovery
for green fluorescent protein (GFPuv). It was found that in the absence of genetic …
for green fluorescent protein (GFPuv). It was found that in the absence of genetic …
Immobilized metal ion affinity chromatography of histidine-tagged fusion proteins
A Charlton, M Zachariou - Affinity Chromatography: Methods and …, 2008 - Springer
Immobilized Metal ion affinity chromatography (IMAC) is a ubiquitous technique in Modern
recombinant production and purification. The wide range of expression vectors for the …
recombinant production and purification. The wide range of expression vectors for the …
Cromatografia de afinidade por íons metálicos imobilizados (IMAC) de biomoléculas: aspectos fundamentais e aplicações tecnológicas
Resumo Immobilized Metal Ion Affinity Cromatography-IMAC-is a group-specific based
adsorption applied to the purification and structure-function studies of proteins and nucleic …
adsorption applied to the purification and structure-function studies of proteins and nucleic …
Immobilized metal affinity membrane separation: characteristics of two materials of differing preparation chemistries
J Crawford, S Ramakrishnan, P Periera… - Separation science …, 1999 - Taylor & Francis
The focus of this research is the investigation of protein retention by two fabricated
membrane modules which differ by a spacing element. Results indicate that both …
membrane modules which differ by a spacing element. Results indicate that both …
Structural damage to lactate dehydrogenase during copper iminodiacetic acid metal affinity chromatography
KD Bush, JA Lumpkin - Biotechnology progress, 1998 - Wiley Online Library
The stability of the enzyme lactate dehydrogenase (LDH) was evaluated by measuring
structural damage and activity loss after exposure to copper− iminodiacetic acid (IDA) …
structural damage and activity loss after exposure to copper− iminodiacetic acid (IDA) …
Immobilized metal ion affinity chromatography of native proteins
A Charlton, M Zachariou - Affinity Chromatography: Methods and …, 2008 - Springer
Immobilized metal affinity chromatography (IMAC) is a common place technique in modern
protein purification. IMAC is distinct from most other affinity chromatography technologies in …
protein purification. IMAC is distinct from most other affinity chromatography technologies in …
Metal-catalyzed oxidation of brain-derived neurotrophic factor (BDNF): Analytical challenges for the identification of modified sites
JL Jensen, C Kolvenbach, S Roy, C Schöneich - Pharmaceutical research, 2000 - Springer
Purpose. We examined the metal-catalyzed oxidation of brain-derivedneurotrophic factor
(BDNF) using the Cu (II)/ascorbate/O 2 modeloxidative system. Methods. Electrospray …
(BDNF) using the Cu (II)/ascorbate/O 2 modeloxidative system. Methods. Electrospray …
Immobilized metal ion affinity chromatography of proteins
M Zachariou - HPLC of Peptides and Proteins: Methods and Protocols, 2004 - Springer
Immobilized metal ion affinity chromatography (IMAC) of proteins is a high-performance
liquid chromatography (HPLC) technique. It has the ability to differentiate a single histidine …
liquid chromatography (HPLC) technique. It has the ability to differentiate a single histidine …