Best practices for single-cell analysis across modalities

L Heumos, AC Schaar, C Lance, A Litinetskaya… - Nature Reviews …, 2023 - nature.com
Recent advances in single-cell technologies have enabled high-throughput molecular
profiling of cells across modalities and locations. Single-cell transcriptomics data can now …

Single‐cell RNA sequencing technologies and applications: A brief overview

D Jovic, X Liang, H Zeng, L Lin, F Xu… - Clinical and …, 2022 - Wiley Online Library
Single‐cell RNA sequencing (scRNA‐seq) technology has become the state‐of‐the‐art
approach for unravelling the heterogeneity and complexity of RNA transcripts within …

Evolutionary-scale prediction of atomic-level protein structure with a language model

Z Lin, H Akin, R Rao, B Hie, Z Zhu, W Lu, N Smetanin… - Science, 2023 - science.org
Recent advances in machine learning have leveraged evolutionary information in multiple
sequence alignments to predict protein structure. We demonstrate direct inference of full …

Personalized RNA neoantigen vaccines stimulate T cells in pancreatic cancer

LA Rojas, Z Sethna, KC Soares, C Olcese, N Pang… - Nature, 2023 - nature.com
Pancreatic ductal adenocarcinoma (PDAC) is lethal in 88% of patients, yet harbours
mutation-derived T cell neoantigens that are suitable for vaccines,. Here in a phase I trial of …

[HTML][HTML] Multiple early factors anticipate post-acute COVID-19 sequelae

Y Su, D Yuan, DG Chen, RH Ng, K Wang, J Choi, S Li… - Cell, 2022 - cell.com
Post-acute sequelae of COVID-19 (PASC) represent an emerging global crisis. However,
quantifiable risk factors for PASC and their biological associations are poorly resolved. We …

An integrated cell atlas of the lung in health and disease

L Sikkema, C Ramírez-Suástegui, DC Strobl… - Nature medicine, 2023 - nature.com
Single-cell technologies have transformed our understanding of human tissues. Yet, studies
typically capture only a limited number of donors and disagree on cell type definitions …

The Tabula Sapiens: A multiple-organ, single-cell transcriptomic atlas of humans

The Tabula Sapiens Consortium*, RC Jones… - Science, 2022 - science.org
Molecular characterization of cell types using single-cell transcriptome sequencing is
revolutionizing cell biology and enabling new insights into the physiology of human organs …

Cross-tissue immune cell analysis reveals tissue-specific features in humans

C Domínguez Conde, C Xu, LB Jarvis, DB Rainbow… - Science, 2022 - science.org
Despite their crucial role in health and disease, our knowledge of immune cells within
human tissues remains limited. We surveyed the immune compartment of 16 tissues from 12 …

Cell2location maps fine-grained cell types in spatial transcriptomics

V Kleshchevnikov, A Shmatko, E Dann… - Nature …, 2022 - nature.com
Spatial transcriptomic technologies promise to resolve cellular wiring diagrams of tissues in
health and disease, but comprehensive map** of cell types in situ remains a challenge …

Liver tumour immune microenvironment subtypes and neutrophil heterogeneity

R Xue, Q Zhang, Q Cao, R Kong, X **ang, H Liu… - Nature, 2022 - nature.com
The heterogeneity of the tumour immune microenvironment (TIME), organized by various
immune and stromal cells, is a major contributing factor of tumour metastasis, relapse and …