This review intends not only to discuss the current possibilities to gain 100% sequence
coverage for proteins, but also to point out the critical limits to such an attempt. The aim of …

The collection of exposed plasma membrane proteins, collectively termed the surfaceome, is
involved in multiple vital cellular processes, such as the communication of cells with their …

We describe a proteomic approach combining the multi-enzyme digestion FASP-sample
processing strategy and the 'Total Protein Approach'applied to absolute quantification of …

Chaperonins are absolutely required for the folding of a subset of proteins in the cell. An
earlier proteome‐wide analysis of Escherichia coli chaperonin GroEL/GroES (GroE) …

We developed a sample preparation protocol for rapid and unbiased analysis of the
membrane proteome using an alimentary canal-mimicking system in which proteases are …

We successfully identified the proteome expressed in Escherichia coli (E. coli) cells on a
microarray scale using one-dimensional capillary liquid chromatography-tandem mass …

Comparative whole-genome sequencing enables the identification of specific mutations
during adaptation of bacteria to new environments and allelic replacement can establish …

Since LC–MS-based quantitative proteomics has become increasingly applied to a wide
range of biological applications over the past decade, numerous studies have performed …

Ganglioside GD 3 is specifically expressed in human melanomas, and plays a role in the
enhancement of malignant phenotypes of melanoma cells. To analyze the mechanisms by …

Site-selective cleavage of the peptide backbone in proteins is an important class of post-
translational modification (PTM) in nature. However, the organic chemistry for such site …