Seed amplification assay for the detection of pathologic alpha-synuclein aggregates in cerebrospinal fluid

L Concha-Marambio, S Pritzkow, M Shahnawaz… - Nature protocols, 2023 - nature.com
Misfolded alpha-synuclein (αSyn) aggregates are a hallmark event in Parkinson's disease
(PD) and other synucleinopathies. Recently, αSyn seed amplification assays (αSyn-SAAs) …

Prions, prionoids and protein misfolding disorders

C Scheckel, A Aguzzi - Nature Reviews Genetics, 2018 - nature.com
Prion diseases are progressive, incurable and fatal neurodegenerative conditions. The term
'prion'was first nominated to express the revolutionary concept that a protein could be …

Development of a biochemical diagnosis of Parkinson disease by detection of α-synuclein misfolded aggregates in cerebrospinal fluid

M Shahnawaz, T Tokuda, M Waragai, N Mendez… - JAMA …, 2017 - jamanetwork.com
Importance Parkinson disease (PD) is a highly prevalent and incurable neurodegenerative
disease associated with the accumulation of misfolded α-synuclein (αSyn) aggregates. An …

Transfusion-related mortality: the ongoing risks of allogeneic blood transfusion and the available strategies for their prevention

EC Vamvakas, MA Blajchman - Blood, The Journal of the …, 2009 - ashpublications.org
As the risks of allogeneic blood transfusion (ABT)–transmitted viruses were reduced to
exceedingly low levels in the US, transfusion-related acute lung injury (TRALI), hemolytic …

Prions: protein aggregation and infectious diseases

A Aguzzi, AM Calella - Physiological reviews, 2009 - journals.physiology.org
Transmissible spongiform encephalopathies (TSEs) are inevitably lethal neurodegenerative
diseases that affect humans and a large variety of animals. The infectious agent responsible …

Rapid end-point quantitation of prion seeding activity with sensitivity comparable to bioassays

JM Wilham, CD Orrú, RA Bessen, R Atarashi… - PLoS …, 2010 - journals.plos.org
A major problem for the effective diagnosis and management of prion diseases is the lack of
rapid high-throughput assays to measure low levels of prions. Such measurements have …

Gold nanorods 3D-supercrystals as surface enhanced Raman scattering spectroscopy substrates for the rapid detection of scrambled prions

RA Alvarez-Puebla, A Agarwal… - Proceedings of the …, 2011 - National Acad Sciences
Highly organized supercrystals of Au nanorods with plasmonic antennae enhancement of
electrical field have made possible fast direct detection of prions in complex biological …

Real time quaking‐induced conversion analysis of cerebrospinal fluid in sporadic Creutzfeldt–Jakob disease

LI McGuire, AH Peden, CD Orrú, JM Wilham… - Annals of …, 2012 - Wiley Online Library
Objective: Current cerebrospinal fluid (CSF) tests for sporadic Creutzfeldt–Jakob disease
(sCJD) are based on the detection of surrogate markers of neuronal damage such as CSF …

The prion's elusive reason for being

A Aguzzi, F Baumann, J Bremer - Annu. Rev. Neurosci., 2008 - annualreviews.org
The protein-only hypothesis posits that the infectious agent causing transmissible
spongiform encephalopathies consists of protein and lacks any informational nucleic acids …

Detection of prions in the plasma of presymptomatic and symptomatic patients with variant Creutzfeldt-Jakob disease

D Bougard, JP Brandel, M Bélondrade… - Science translational …, 2016 - science.org
Variant Creutzfeldt-Jakob disease (vCJD) is a human prion disease resulting from the
consumption of meat products contaminated by the agent causing bovine spongiform …