[HTML][HTML] Environmental DNA metabarcoding for benthic monitoring: A review of sediment sampling and DNA extraction methods

J Pawlowski, K Bruce, K Panksep, FI Aguirre… - Science of the Total …, 2022 - Elsevier
Environmental DNA (eDNA) metabarcoding (parallel sequencing of DNA/RNA for
identification of whole communities within a targeted group) is revolutionizing the field of …

Ecosystems monitoring powered by environmental genomics: a review of current strategies with an implementation roadmap

T Cordier, L Alonso‐Sáez… - Molecular …, 2021 - Wiley Online Library
A decade after environmental scientists integrated high‐throughput sequencing
technologies in their toolbox, the genomics‐based monitoring of anthropogenic impacts on …

The ecologist's field guide to sequence‐based identification of biodiversity

S Creer, K Deiner, S Frey, D Porazinska… - Methods in ecology …, 2016 - Wiley Online Library
The past 100 years of ecological research has seen substantial progress in understanding
the natural world and likely effects of change, whether natural or anthropogenic. Traditional …

Scaling up: A guide to high‐throughput genomic approaches for biodiversity analysis

TM Porter, M Hajibabaei - Molecular ecology, 2018 - Wiley Online Library
The purpose of this review is to present the most common and emerging DNA‐based
methods used to generate data for biodiversity and biomonitoring studies. As environmental …

Towards next‐generation biodiversity assessment using DNA metabarcoding

P Taberlet, E Coissac, F Pompanon… - Molecular …, 2012 - Wiley Online Library
Virtually all empirical ecological studies require species identification during data collection.
DNA metabarcoding refers to the automated identification of multiple species from a single …

Next‐generation sequencing technologies for environmental DNA research

S Shokralla, JL Spall, JF Gibson… - Molecular …, 2012 - Wiley Online Library
Since 2005, advances in next‐generation sequencing technologies have revolutionized
biological science. The analysis of environmental DNA through the use of specific gene …

Environmental DNA metabarcoding of lake fish communities reflects long‐term data from established survey methods

B Hänfling, L Lawson Handley, DS Read… - Molecular …, 2016 - Wiley Online Library
Organisms continuously release DNA into their environments via shed cells, excreta,
gametes and decaying material. Analysis of this 'environmental DNA'(eDNA) is …

Characterization of the 18S rRNA gene for designing universal eukaryote specific primers

K Hadziavdic, K Lekang, A Lanzen, I Jonassen… - PloS one, 2014 - journals.plos.org
High throughput sequencing technology has great promise for biodiversity studies.
However, an underlying assumption is that the primers used in these studies are universal …

Biodiversity soup: metabarcoding of arthropods for rapid biodiversity assessment and biomonitoring

DW Yu, Y Ji, BC Emerson, X Wang, C Ye… - Methods in Ecology …, 2012 - Wiley Online Library
Traditional biodiversity assessment is costly in time, money and taxonomic expertise.
Moreover, data are frequently collected in ways (eg visual bird lists) that are unsuitable for …

Tree diversity and species identity effects on soil fungi, protists and animals are context dependent

L Tedersoo, M Bahram, T Cajthaml, S Põlme… - The ISME …, 2016 - academic.oup.com
Plant species richness and the presence of certain influential species (sampling effect) drive
the stability and functionality of ecosystems as well as primary production and biomass of …