Past, present and future of ICSI in livestock species

O Briski, DF Salamone - Animal Reproduction Science, 2022 - Elsevier
During the past 2 decades, intracytoplasmic sperm injection (ICSI) has become a routine
technique for clinical applications in humans. The widespread use among domestic species …

Intracytoplasmic sperm injection in domestic and wild mammals

DF Salamone, NG Canel, MB Rodríguez - Reproduction, 2017 - rep.bioscientifica.com
Intracytoplasmic sperm injection (ICSI) has become a useful technique for clinical
applications in the horse-breeding industry. However, both ICSI blastocyst and offspring …

Bovine blastocyst development from oocytes injected with freeze-dried spermatozoa

L Keskintepe, G Pacholczyk, A Machnicka… - Biology of …, 2002 - academic.oup.com
Pronuclear formation, and the chromosomal constitution and developmental capacity of
bovine zygotes formed by intracytoplasmic sperm injection with freeze-dried (lyophilized) …

Sperm and oocyte treatments to improve the formation of male and female pronuclei and subsequent development following intracytoplasmic sperm injection into …

GJ Rho, S Kawarsky, WH Johnson… - Biology of …, 1998 - academic.oup.com
This study assessed pronuclear formation, the chromosomal constitution, and the
developmental capacity of bovine zygotes formed by intracytoplasmic injection of oocytes …

Activation, pronuclear formation, and development in vitro of pig oocytes following intracytoplasmic injection of freeze-dried spermatozoa

IK Kwon, KE Park, K Niwa - Biology of Reproduction, 2004 - academic.oup.com
The fertilization of pig oocytes following intracytoplasmic injection of freeze-dried
spermatozoa was evaluated. Activation and male pronuclear (MPN) formation were better in …

Intracytoplasmic sperm injection in bovine: effects of oocyte activation, sperm pretreatment and injection technique

R Suttner, V Zakhartchenko, P Stojkovic, S Müller… - Theriogenology, 2000 - Elsevier
Intracytoplasmic sperm injection (ICSI) is a very important technique for treating male
subfertility and for basic research. The efficiency of ICSI in bovine is very limited because of …

Intracytoplasmic sperm injection in livestock species: an update

E García‐Roselló, E García‐Mengual… - … in Domestic Animals, 2009 - Wiley Online Library
Contents Intracytoplasmic sperm injection (ICSI) is a powerful technique in the field of
assisted reproduction (ART) and provides exciting opportunities for studying the basic …

Bovine ICSI: Limiting factors, strategies to improve its efficiency and alternative approaches

F Fuentes, E Munoz, MJ Contreras, ME Arias, R Felmer - Zygote, 2022 - cambridge.org
Intracytoplasmic sperm injection (ICSI) is an assisted reproductive technique mainly used to
overcome severe infertility problems associated with the male factor, but in cattle its …

Development of goat embryos after in vitro fertilization and parthenogenetic activation by different methods

EM Ongeri, CL Bormann, RE Butler, D Melican… - Theriogenology, 2001 - Elsevier
Effective activation protocols that can be used during nuclear transfer investigations in goats
need to be developed. We compared the development of IVF goat embryos with those of …

Effects of different activation protocols on preimplantation development, apoptosis and ploidy of bovine parthenogenetic embryos

Z Wang, W Wang, S Yu, Z Xu - Animal reproduction science, 2008 - Elsevier
The objective of this study was to optimize the protocols for bovine oocytes activation
through comparing the effectiveness of different treatments on the activation and subsequent …