Background Technologies for making and manipulating DNA have enabled advances in
biology ever since the discovery of the DNA double helix. But introducing site-specific …

The CRISPR/Cas9 system is a rapid, simple, and often extremely efficient gene editing
method. This method has been used in a variety of organisms and cell types over the past …

Major advances in genome editing have recently been made possible with the development
of the TALEN and CRISPR/Cas9 methods. The speed and ease of implementing these …

Current technology enables the production of highly specific genome modifications with
excellent efficiency and specificity. Key to this capability are targetable DNA cleavage …

Introgression of farmed salmon escapees into wild stocks is a major threat to the genetic
integrity of wild populations. Using germ cell-free fish in aquaculture may mitigate this …

Tyrosinase is the rate-limiting enzyme for the production of melanin pigmentation. In the
mouse and other animals, homozygous null mutations in the Tyrosinase gene (Tyr) result in …

We have assessed the efficacy of the recently developed CRISPR/Cas (clustered regularly
interspaced short palindromic repeats/CRISPR‐associated) system for genome modification …

The CRISPR (clustered regularly interspaced short palindromic repeat)–Cas9 (CRISPR-
associated nuclease 9) system is poised to transform developmental biology by providing a …

Understanding the biological function behind key proteins is of great concern in Atlantic
salmon, both due to a high commercial importance and an interesting life history. Until …

The diverse applications of genetically modified cells and organisms require more precise
and efficient genome-editing tool such as clustered regularly interspaced short palindromic …