Microfluidic‐based approaches in targeted cell/particle separation based on physical properties: fundamentals and applications

R Nasiri, A Shamloo, S Ahadian, L Amirifar, J Akbari… - Small, 2020 - Wiley Online Library
Cell separation is a key step in many biomedical research areas including biotechnology,
cancer research, regenerative medicine, and drug discovery. While conventional cell sorting …

Revisiting 30 years of biofunctionalization and surface chemistry of inorganic nanoparticles for nanomedicine

J Conde, JT Dias, V Grazú, M Moros… - Frontiers in …, 2014 - frontiersin.org
In the last 30 years we have assisted to a massive advance of nanomaterials in material
science. Nanomaterials and structures, in addition to their small size, have properties that …

Fundamentals and application of magnetic particles in cell isolation and enrichment: a review

BD Plouffe, SK Murthy, LH Lewis - Reports on Progress in …, 2014 - iopscience.iop.org
Magnetic sorting using magnetic beads has become a routine methodology for the
separation of key cell populations from biological suspensions. Due to the inherent ability of …

High gradient magnetic cell separation with MACS

S Miltenyi, W Müller, W Weichel… - Cytometry: The Journal …, 1990 - Wiley Online Library
A flexible, fast and simple magnetic cell sorting system for separation of large numbers of
cells according to specific cell surface markers was developed and tested. Cells stained …

Immunospecific ferromagnetic iron-dextran reagents for the labeling and magnetic separation of cells

RS Molday, D Mackenzie - Journal of immunological methods, 1982 - Elsevier
Ferromagnetic iron dextran particles were prepared by reacting a mixture of ferrous chloride
and ferric chloride with dextran polymers under alkaline conditions. Particles purified by gel …

Magnetoferritin: in vitro synthesis of a novel magnetic protein

FC Meldrum, BR Heywood, S Mann - Science, 1992 - science.org
The iron storage protein ferritin consists of a spherical polypeptide shell (apoferritin)
surrounding a 6-nanometer inorganic core of the hydrated iron oxide ferrihydrite (5Fe2O3⋅ …

Magnetic separations: from steel plants to biotechnology

CT Yavuz, A Prakash, JT Mayo, VL Colvin - Chemical Engineering Science, 2009 - Elsevier
Magnetic separations have for decades been essential processes in diverse industries
ranging from steel production to coal desulfurization. In such settings magnetic fields are …

Magnetic separations in biotechnology

L Borlido, AM Azevedo, ACA Roque… - Biotechnology …, 2013 - Elsevier
Magnetic separations are probably one of the most versatile separation processes in
biotechnology as they are able to purify cells, viruses, proteins and nucleic acids directly …

Antigen-specific identification and cloning of hybridomas with a fluorescence-activated cell sorter.

DR Parks, VM Bryan, VT Oi, LA Herzenberg - Proceedings of the National …, 1979 - pnas.org
Myeloma-spleen cell hybrids (hybridomas) producing antibody to mouse immunoglobulin
allotypes have been labeled with fluorescent microspheres coupled with myeloma protein …

Microfluidic: an innovative tool for efficient cell sorting

J Autebert, B Coudert, FC Bidard, JY Pierga… - Methods, 2012 - Elsevier
At first mostly dedicated to molecular analysis, microfluidic systems are rapidly expanding
their range of applications towards cell biology, thanks to their ability to control the …