The βγ-crystallins: Native state stability and pathways to aggregation

E Serebryany, JA King - Progress in biophysics and molecular biology, 2014 - Elsevier
The βγ-crystallins are among the most stable and long-lived proteins in the human body.
With increasing age, however, they transform to high molecular weight light-scattering …

Oxidative stress and antioxidant-based interventional medicine in ophthalmology

C Honisch, U Rodella, C Gatto, P Ruzza, JDA Tóthová - Pharmaceuticals, 2023 - mdpi.com
The different anatomical compartments of the eye are highly subjected to reactive oxygen
species (ROS) generation due to internal factors, such as metabolic high oxygen …

Lanosterol disrupts aggregation of human γD-crystallin by binding to the hydrophobic dimerization interface

H Kang, Z Yang, R Zhou - Journal of the American Chemical …, 2018 - ACS Publications
Cataracts are a leading cause of vision impairment, which stem from the misfolding and
aggregation of crystallins in the eye lens. Despite its prevalence and severity, the detailed …

Aggregation of Trp> Glu point mutants of human gamma‐D crystallin provides a model for hereditary or UV‐induced cataract

E Serebryany, T Takata, E Erickson… - Protein …, 2016 - Wiley Online Library
Numerous mutations and covalent modifications of the highly abundant, long‐lived
crystallins of the eye lens cause their aggregation leading to progressive opacification of the …

Phototoxicity of environmental radiations in human lens: Revisiting the pathogenesis of UV-induced cataract

F Kamari, S Hallaj, F Dorosti, F Alinezhad… - Graefe's Archive for …, 2019 - Springer
The magnitude of cataract pathology is indeed significant as it is the principal cause of
blindness worldwide. Also, the prominence of this concept escalates with the current aging …

[HTML][HTML] Reactive cysteine residues in the oxidative dimerization and Cu2+ induced aggregation of human γD-crystallin: Implications for age-related cataract

S Ramkumar, X Fan, B Wang, S Yang… - Biochimica et Biophysica …, 2018 - Elsevier
Cysteine (Cys) residues are major causes of crystallin disulfide formation and aggregation in
aging and cataractous human lenses. We recently found that disulfide linkages are highly …

Cataract-prone variants of γD-crystallin populate a conformation with a partially unfolded N-terminal domain under native conditions

S Volz, JR Malone, AJ Guseman… - Proceedings of the …, 2025 - pnas.org
Human γD-crystallin, a monomeric protein abundant in the eye lens nucleus, must remain
stably folded for an individual's entire lifetime to avoid aggregation and protein deposition …

Emerging β-sheet rich conformations in supercompact huntingtin exon-1 mutant structures

H Kang, FX Vázquez, L Zhang, P Das… - Journal of the …, 2017 - ACS Publications
There exists strong correlation between the extended polyglutamines (polyQ) within exon-1
of Huntingtin protein (Htt) and age onset of Huntington's disease (HD); however, the …

Amyloid fiber formation in human γD-crystallin induced by UV–B photodamage

SD Moran, TO Zhang, SM Decatur, MT Zanni - Biochemistry, 2013 - ACS Publications
γD-Crystallin is an abundant structural protein of the lens that is found in native and modified
forms in cataractous aggregates. We establish that UV–B irradiation of γD-Crystallin leads to …

Amyloid β-sheet secondary structure identified in UV-induced cataracts of porcine lenses using 2D IR spectroscopy

TO Zhang, AM Alperstein, MT Zanni - Journal of molecular biology, 2017 - Elsevier
Cataracts are formed by the aggregation of crystallin proteins in the eye lens. Many in vitro
studies have established that crystallin proteins precipitate into aggregates that contain …