Data analysis guidelines for single-cell RNA-seq in biomedical studies and clinical applications

M Su, T Pan, QZ Chen, WW Zhou, Y Gong, G Xu… - Military Medical …, 2022 - Springer
The application of single-cell RNA sequencing (scRNA-seq) in biomedical research has
advanced our understanding of the pathogenesis of disease and provided valuable insights …

Decoding aging hallmarks at the single-cell level

S Ma, X Chi, Y Cai, Z Ji, S Wang, J Ren… - Annual Review of …, 2023 - annualreviews.org
Organismal aging exhibits wide-ranging hallmarks in divergent cell types across tissues,
organs, and systems. The advancement of single-cell technologies and generation of rich …

Inferring neuron-neuron communications from single-cell transcriptomics through NeuronChat

W Zhao, KG Johnston, H Ren, X Xu, Q Nie - Nature communications, 2023 - nature.com
Neural communication networks form the fundamental basis for brain function. These
communication networks are enabled by emitted ligands such as neurotransmitters, which …

Spatial transcriptomic analysis of primary and metastatic pancreatic cancers highlights tumor microenvironmental heterogeneity

AM Khaliq, M Rajamohan, O Saeed, K Mansouri… - Nature Genetics, 2024 - nature.com
Although the spatial, cellular and molecular landscapes of resected pancreatic ductal
adenocarcinoma (PDAC) are well documented, the characteristics of its metastatic ecology …

Systems-based approaches to study immunometabolism

V Purohit, A Wagner, N Yosef… - Cellular & molecular …, 2022 - nature.com
Technical advances at the interface of biology and computation, such as single-cell RNA-
sequencing (scRNA-seq), reveal new layers of complexity in cellular systems. An emerging …

Targeting neoadjuvant chemotherapy-induced metabolic reprogramming in pancreatic cancer promotes anti-tumor immunity and chemo-response

R Tang, J Xu, W Wang, Q Meng, C Shao, Y Zhang… - Cell Reports …, 2023 - cell.com
The molecular dynamics of pancreatic ductal adenocarcinoma (PDAC) under chemotherapy
remain incompletely understood. The widespread use of neoadjuvant chemotherapy (NAC) …

Generation and analysis of context-specific genome-scale metabolic models derived from single-cell RNA-Seq data

J Gustafsson, M Anton… - Proceedings of the …, 2023 - National Acad Sciences
Single-cell RNA sequencing combined with genome-scale metabolic models (GEMs) has
the potential to unravel the differences in metabolism across both cell types and cell states …

Interpretable machine learning methods for predictions in systems biology from omics data

D Sidak, J Schwarzerová, W Weckwerth… - Frontiers in molecular …, 2022 - frontiersin.org
Machine learning has become a powerful tool for systems biologists, from diagnosing
cancer to optimizing kinetic models and predicting the state, growth dynamics, or type of a …

[HTML][HTML] Toward modeling metabolic state from single-cell transcriptomics

K Hrovatin, DS Fischer, FJ Theis - Molecular Metabolism, 2022 - Elsevier
Background Single-cell metabolic studies bring new insights into cellular function, which can
often not be captured on other omics layers. Metabolic information has wide applicability …

Single‐cell and spatial analyses reveal the association between gene expression of glutamine synthetase with the immunosuppressive phenotype of APOE+ CTSZ+ …

J Wei, W Yu, J Chen, G Huang, L Zhang… - Molecular …, 2023 - Wiley Online Library
An immunosuppressive state is regulated by various factors in the tumor microenvironment
(TME), including, but not limited to, metabolic plasticity of immunosuppressive cells and …