Column characterization and selection systems in reversed-phase high-performance liquid chromatography

P Žuvela, M Skoczylas, J Jay Liu, T Ba̧czek… - Chemical …, 2019 - ACS Publications
Reversed-phase high-performance liquid chromatography (RP-HPLC) is the most popular
chromatographic mode, accounting for more than 90% of all separations. HPLC itself owes …

Hydrophilic interaction liquid chromatography (HILIC)—a powerful separation technique

B Buszewski, S Noga - Analytical and bioanalytical chemistry, 2012 - Springer
Hydrophilic interaction liquid chromatography (HILIC) provides an alternative approach to
effectively separate small polar compounds on polar stationary phases. The purpose of this …

Stationary and mobile phases in hydrophilic interaction chromatography: a review

P Jandera - Analytica chimica acta, 2011 - Elsevier
Hydrophilic interaction chromatography (HILIC) is valuable alternative to reversed-phase
liquid chromatography separations of polar, weakly acidic or basic samples. In principle, this …

Hydrophilic interaction chromatography

P Hemström, K Irgum - Journal of separation science, 2006 - Wiley Online Library
Separation of polar compounds on polar stationary phases with partly aqueous eluents is by
no means a new separation mode in LC. The first HPLC applications were published more …

Glycan labeling strategies and their use in identification and quantification

LR Ruhaak, G Zauner, C Huhn, C Bruggink… - Analytical and …, 2010 - Springer
Most methods for the analysis of oligosaccharides from biological sources require a glycan
derivatization step: glycans may be derivatized to introduce a chromophore or fluorophore …

High-sensitivity analytical approaches for the structural characterization of glycoproteins

WR Alley Jr, BF Mann, MV Novotny - Chemical reviews, 2013 - ACS Publications
1.1. General Considerations Structural intricacies of carbohydrate molecules and their
propensity to form varied linkages, substitutions, and branching patterns have fascinated …

Electrostatic repulsion hydrophilic interaction chromatography for isocratic separation of charged solutes and selective isolation of phosphopeptides

AJ Alpert - Analytical chemistry, 2008 - ACS Publications
If an ion-exchange column is eluted with a predominantly organic mobile phase, then
solutes can be retained through hydrophilic interaction even if they have the same charge as …

Retention and selectivity of stationary phases for hydrophilic interaction chromatography

Y Guo, S Gaiki - Journal of Chromatography A, 2011 - Elsevier
More and more polar stationary phases have become available for the separation of small
polar compounds in the past decade as hydrophilic interaction chromatography (HILIC) …

Retention behavior of small polar compounds on polar stationary phases in hydrophilic interaction chromatography

Y Guo, S Gaiki - Journal of Chromatography A, 2005 - Elsevier
Retention characteristics of four polar stationary phases (ie, amide, amino, silica and
sulfobetaine) were studied by using a group of small polar compounds in hydrophilic …

Analysis of highly polar compounds of plant origin: combination of hydrophilic interaction chromatography and electrospray ion trap mass spectrometry

VV Tolstikov, O Fiehn - Analytical biochemistry, 2002 - Elsevier
The primary goal of metabolomic analysis is the unbiased relative quantification of every
metabolite in a biological system. A number of different metabolite-profiling techniques must …