The study of biomolecules in their native environments is a challenging task because of the
vast complexity of cellular systems. Technologies developed in the last few years for the …

The development of new orthogonal aminoacyl-tRNA synthetase/tRNA pairs has led to the
addition of approximately 70 unnatural amino acids (UAAs) to the genetic codes of …

Protein-based conjugates are valuable constructs for a variety of applications. Conjugation
of proteins to fluorophores is commonly used to study their cellular localization and the …

The genetic incorporation of the 22nd proteinogenic amino acid, pyrrolysine (Pyl) at amber
codon is achieved by the action of pyrrolysyl-tRNA synthetase (PylRS) together with its …

Genome‐modification technologies enable the rational engineering and perturbation of
biological systems. Historically, these methods have been limited to gene insertions or …

Protein-protein interactions are the key to organizing cellular processes in space and time.
The only direct way to identify such interactions in their cellular environment is by photo …

From its start as a small‐scale in vitro system to study fundamental translation processes,
cell‐free protein synthesis quickly rose to become a potent platform for the high‐yield …

In recent years new nucleic acid and protein‐based combinatorial molecules have attracted
the attention of researchers working in various areas of science, ranging from medicine to …

Mechanisms of protein misfolding are of increasing interest in the aetiology of
neurodegenerative diseases characterized by protein aggregation and tangles including …

Non-natural amino acids are increasingly used as building blocks in the development of
peptide-based drugs as they expand the available chemical space to tailor function, half-life …