Spatially resolved mass spectrometry at the single cell: recent innovations in proteomics and metabolomics
Biological systems are composed of heterogeneous populations of cells that
intercommunicate to form a functional living tissue. Biological function varies greatly across …
intercommunicate to form a functional living tissue. Biological function varies greatly across …
Recent advances in robotic protein sample preparation for clinical analysis and other biomedical applications
Discovery of new protein biomarker candidates has become a major research goal in the
areas of clinical chemistry, analytical chemistry, and biomedicine. These important species …
areas of clinical chemistry, analytical chemistry, and biomedicine. These important species …
Simple Tool for Rapidly Assessing the Quality of Multiplexed Single Cell Proteomics Data
C Jenkins, BC Orsburn - Journal of the American Society for Mass …, 2023 - ACS Publications
Recent advances in the sensitivity and speed of mass spectrometers coupled with improved
sample preparation methods have enabled the field of single cell proteomics to proliferate …
sample preparation methods have enabled the field of single cell proteomics to proliferate …
[HTML][HTML] Evaluation of the sensitivity of proteomics methods using the absolute copy number of proteins in a single cell as a metric
BC Orsburn - Proteomes, 2021 - mdpi.com
Proteomic technology has improved at a staggering pace in recent years, with even
practitioners challenged to keep up with new methods and hardware. The most common …
practitioners challenged to keep up with new methods and hardware. The most common …
Rapid multivariate analysis approach to explore differential spatial protein profiles in tissue
Spatially targeted proteomics analyzes the proteome of specific cell types and functional
regions within tissue. While spatial context is often essential to understanding biological …
regions within tissue. While spatial context is often essential to understanding biological …
Time-of-flight fragmentation spectra generated by the proteomic analysis of single human cells do not exhibit atypical fragmentation patterns
BC Orsburn - Journal of proteome research, 2023 - ACS Publications
Recent work detailed the unique characteristics of fragmentation spectra derived from
peptides from single human cells. This valuable report utilized an ultrahigh-field Orbitrap …
peptides from single human cells. This valuable report utilized an ultrahigh-field Orbitrap …
[HTML][HTML] Acetic acid is a superior acidifier for sub-nanogram and single cell proteomic studies
BC Orsburn - bioRxiv, 2023 - ncbi.nlm.nih.gov
A recent study demonstrated a substantial signal increase when employing a 0.5% acetic
acid buffer additive instead of the traditional 0.1% formic acid used in shotgun proteomics. In …
acid buffer additive instead of the traditional 0.1% formic acid used in shotgun proteomics. In …
Parallel sample processing for mass spectrometry-based single cell proteomics
Background Single cell mass spectrometry (scMS) has shown great promise for label free
proteomics analysis recently. To present single cell samples for proteomics analysis by MS …
proteomics analysis recently. To present single cell samples for proteomics analysis by MS …
[HTML][HTML] An integrated method for single cell proteomics with simultaneous measurements of intracellular drug concentration implicates new mechanisms for …
BC Orsburn - bioRxiv, 2023 - ncbi.nlm.nih.gov
It is well established that a population of single human cells will often respond to the same
drug treatment in a heterogeneous manner. In the context of chemotherapeutics, these …
drug treatment in a heterogeneous manner. In the context of chemotherapeutics, these …
[HTML][HTML] SCP Viz–A universal graphical user interface for single protein analysis in single cell proteomics datasets
A Warshanna, BC Orsburn - bioRxiv, 2023 - ncbi.nlm.nih.gov
Single cell proteomics (SCP) requires the analysis of dozens to thousands of single human
cells to draw biological conclusions. However, assessing of the abundance of single …
cells to draw biological conclusions. However, assessing of the abundance of single …