Interfacing data science with cell therapy manufacturing: Where we are and where we need to be

K Roy, B Wang, RQ Chen, J Li - Cytotherapy, 2024 - Elsevier
Although several cell-based therapies have received FDA approval, and others are showing
promising results, scalable, and quality-driven reproducible manufacturing of therapeutic …

Analysis of structural effects of sickle cell disease on brain vasculature of mice using three-dimensional quantitative phase imaging

CE Serafini, H Song, MO Platt… - Journal of Biomedical …, 2023 - spiedigitallibrary.org
Significance Although the molecular origins of sickle cell disease (SCD) have been
extensively studied, the effects of SCD on the vasculature—which can influence blood …

Real-time semantic segmentation and anomaly detection of functional images for cell therapy manufacturing

RQ Chen, B Joffe, PC Costa, C Filan, B Wang… - Cytotherapy, 2023 - Elsevier
Background aims Cell therapy is a promising treatment method that uses living cells to
address a variety of diseases and conditions, including cardiovascular diseases, neurologic …

Non-invasive label-free imaging analysis pipeline for in situ characterization of 3D brain organoids

CE Serafini, S Charles, P Casteleiro Costa, W Niu… - Scientific Reports, 2024 - nature.com
Brain organoids provide a unique opportunity to model organ development in a system
similar to human organogenesis in vivo. Brain organoids thus hold great promise for drug …

Non-invasive label-free analysis pipeline for in situ characterization of differentiation in 3d brain organoid models

C Filan, S Charles, PC Costa, W Niu… - Research …, 2024 - pmc.ncbi.nlm.nih.gov
Brain organoids provide a unique opportunity to model organ development in a system
similar to human organogenesis in vivo. Brain organoids thus hold great promise for drug …

SLIM-assisted automatic cartography of cell death types and rates resulting from localized photodynamic treatment

AV Belashov, AA Zhikhoreva, AV Salova… - Journal of the Optical …, 2024 - opg.optica.org
We report a spatial light interference microscopy (SLIM)-based methodology aimed at
automatic monitoring and analysis of changes in cellular morphology within extended fields …

Label-free functional analysis of root-associated microbes with dynamic quantitative oblique back-illumination microscopy

C Filan, M Green, A Diering, MT Cicerone… - Scientific Reports, 2024 - nature.com
The increasing global demand for food, coupled with concerns about the environmental
impact of synthetic fertilizers, underscores the urgency of develo** sustainable agricultural …

Acetic acid enabled nuclear contrast enhancement in epi-mode quantitative phase imaging

Z Guang, A Jacobs, PC Costa, Z Li… - Journal of Biomedical …, 2025 - spiedigitallibrary.org
Significance The acetowhitening effect of acetic acid (AA) enhances light scattering of cell
nuclei, an effect that has been widely leveraged to facilitate tissue inspection for (pre) …

Experimental assessment of the optical transfer function for quantitative oblique back illumination microscopy (qOBM)

Z Li, P Casteleiro Costa, C Serafini, S Bharadwaj… - Optics …, 2025 - opg.optica.org
Quantitative oblique back illumination microscopy (qOBM) is a recently developed imaging
technique that enables 3D quantitative phase imaging (QPI) and refractive index (RI) …

Gradient Optical Diffraction Tomography

J Winnik, P Zdankowski, M Stefaniuk, A Ahmad… - arxiv preprint arxiv …, 2024 - arxiv.org
Optical diffraction tomography (ODT) enables non-invasive information-rich 3D refractive
index (RI) reconstruction of unimpaired transparent biological and technical samples, crucial …