Recent advances in instrumentation and software have resulted in cryo-EM rapidly
becoming the method of choice for structural biologists, especially for those studying the …

The recent rapid development of single-particle electron cryo-microscopy (cryo-EM) now
allows structures to be solved by this method at resolutions close to 3 Å. Here, a number of …

The identification and exploration of (dis) similarities between macromolecular structures
can help to gain biological insight, for instance when visualizing or quantifying the response …

Highlights•Crystal handling to reduce damage, variations and background.•Beamline
instrumentation and data collection strategies for weak anomalous signals.•High multiplicity …

In this contribution, the current protocols for modelling covalent linkages within the CCP4
suite are considered. The mechanism used for modelling covalent linkages is reviewed: the …

A recent bioinformatic analysis of well-characterized classes of riboswitches uncovered
subgroups unable to bind to the regulatory molecule of the parental class. Within the …

The refinement of macromolecular structures is usually aided by prior stereochemical
knowledge in the form of geometrical restraints. Such restraints are also used for the flexible …

The mammalian brain dynamically activates or silences gene programs in response to
environmental input and developmental cues. This neuroplasticity is controlled by signaling …

The maltooligosaccharide (MOS) utilization locus in Lactobacillus acidophilus NCFM, a
model for human small-intestine lactobacilli, encodes three glycoside hydrolases (GHs): a …

In an effort to evaluate whether a recently reported putative metallo-β-lactamase (MβL)
contains a novel MβL active site, SPS-1 from Sediminispirochaeta smaragdinae was …