Optical tweezers in single-molecule biophysics
Optical tweezers have become the method of choice in single-molecule manipulation
studies. In this Primer, we first review the physical principles of optical tweezers and the …
studies. In this Primer, we first review the physical principles of optical tweezers and the …
An overview of the molecular mechanisms of recombinational DNA repair
SC Kowalczykowski - Cold Spring Harbor Perspectives …, 2015 - cshperspectives.cshlp.org
Recombinational DNA repair is a universal aspect of DNA metabolism and is essential for
genomic integrity. It is a template-directed process that uses a second chromosomal copy …
genomic integrity. It is a template-directed process that uses a second chromosomal copy …
The human Shu complex promotes RAD51 activity by modulating RPA dynamics on ssDNA
Templated DNA repair that occurs during homologous recombination and replication stress
relies on RAD51. RAD51 activity is positively regulated by BRCA2 and the RAD51 paralogs …
relies on RAD51. RAD51 activity is positively regulated by BRCA2 and the RAD51 paralogs …
Visualization of direct and diffusion-assisted RAD51 nucleation by full-length human BRCA2 protein
Homologous recombination (HR) is essential for error-free repair of DNA double-strand
breaks, perturbed replication forks (RFs), and post-replicative single-stranded DNA (ssDNA) …
breaks, perturbed replication forks (RFs), and post-replicative single-stranded DNA (ssDNA) …
Protein dynamics of human RPA and RAD51 on ssDNA during assembly and disassembly of the RAD51 filament
Homologous recombination (HR) is a crucial pathway for double-stranded DNA break (DSB)
repair. During the early stages of HR, the newly generated DSB ends are processed to yield …
repair. During the early stages of HR, the newly generated DSB ends are processed to yield …
Exploiting DNA repair defects in colorectal cancer
NM Reilly, L Novara, F Di Nicolantonio… - Molecular …, 2019 - Wiley Online Library
Colorectal cancer (CRC) is the third leading cause of cancer‐related deaths worldwide.
Therapies that take advantage of defects in DNA repair pathways have been explored in the …
Therapies that take advantage of defects in DNA repair pathways have been explored in the …
Dynamics and selective remodeling of the DNA-binding domains of RPA
Replication protein A (RPA) coordinates important DNA metabolic events by stabilizing
single-stranded DNA (ssDNA) intermediates, activating the DNA-damage response and …
single-stranded DNA (ssDNA) intermediates, activating the DNA-damage response and …
Single-molecule analysis reveals cooperative stimulation of Rad51 filament nucleation and growth by mediator proteins
Homologous recombination (HR) is an essential DNA double-strand break (DSB) repair
mechanism, which is frequently inactivated in cancer. During HR, RAD51 forms …
mechanism, which is frequently inactivated in cancer. During HR, RAD51 forms …
Co-condensation of proteins with single-and double-stranded DNA
R Renger, JA Morin, R Lemaitre… - Proceedings of the …, 2022 - National Acad Sciences
Biomolecular condensates provide distinct compartments that can localize and organize
biochemistry inside cells. Recent evidence suggests that condensate formation is prevalent …
biochemistry inside cells. Recent evidence suggests that condensate formation is prevalent …
Nucleotide binding halts diffusion of the eukaryotic replicative helicase during activation
The eukaryotic replicative helicase CMG centrally orchestrates the replisome and leads the
way at the front of replication forks. Understanding the motion of CMG on the DNA is …
way at the front of replication forks. Understanding the motion of CMG on the DNA is …