Cell manipulation in droplets has emerged as one of the great successes of microfluidic
technologies, with the development of single-cell screening. However, the droplet format has …

During the last decade, advances in single cell technologies have ignited increased
understanding of natural killer cells (NK cells), which turned out to be far more complex than …

A microfluidic-based surface-enhanced Raman scattering (SERS) platform for analyzing
cytokines secreted by single cells is reported based on the elaborate bioconjugation of the …

Monitoring cell secretion in complex microenvironments is crucial for understanding cellular
behavior and advancing physiological and pathological research. While traditional cell …

Telomerase is a significant biomarker for potential early cancer diagnosis and therapy.
Exploring telomerase activity in single cells presents great challenges due to the complexity …

Screening T-cell activity and selecting active ones from large ex vivo-expanded populations
before reinfusion is important for the success of T-cell therapy. Cytokine secretion is the …

Despite several extraordinary improvements in cancer immunotherapy, its therapeutic
effectiveness against many distinct cancer types remains mostly limited and requires further …

Cytotoxic T-cells (CTLs) exhibit strong effector functions to leverage antigen-specific anti-
tumoral and anti-viral immunity. When naïve CTLs are activated by antigen-presenting cells …

Single-cell techniques have become more and more incorporated in cell biological research
over the past decades. Various approaches have been proposed to isolate, culture, sort and …

The Poisson limit is a major problem for the isolation of single cells in different single-cell
technologies and applications. In droplet-based single-cell assays, a scheme that is …