Non-covalent capillary coatings for protein separations in capillary electrophoresis

CA Lucy, AM MacDonald, MD Gulcev - Journal of Chromatography A, 2008 - Elsevier
Adsorption of proteins, particularly basic proteins onto fused silica capillaries severely
degrades capillary electrophoretic performance. This review provides a synopsis of the …

Microchannel wall coatings for protein separations by capillary and chip electrophoresis

EAS Doherty, RJ Meagher, MN Albarghouthi… - …, 2003 - Wiley Online Library
The necessity for microchannel wall coatings in capillary and chip‐based electrophoretic
analysis of biomolecules is well understood. The regulation or elimination of EOF and the …

The state of the art of dynamic coatings

PG Righetti, C Gelfi, B Verzola, L Castelletti - Electrophoresis, 2001 - Wiley Online Library
The present review highlights the mechanisms of action and efficiency of three major
classes of dynamic coatings so far adopted in capillary electrophoresis:(i) amines to oligo …

Protein attachment onto silica surfaces–a survey of molecular fundamentals, resulting effects and novel preventive strategies in CE

H Stutz - Electrophoresis, 2009 - Wiley Online Library
This review addresses the fundamentals governing the adsorption of individual protein
molecules onto the surface of fused‐silica capillaries, the protein aggregation to adsorbate …

Strategies for capillary electrophoresis: method development and validation for pharmaceutical and biological applications

H Wätzig, M Degenhardt, A Kunkel - Electrophoresis, 1998 - Wiley Online Library
This review is in support of the development of selective, reproducible and validated
capillary electrophoretis (CE) methods. Focusing on pharmaceutical and biological …

Protein adsorption to the bare silica wall in capillary electrophoresis: Quantitative study on the chemical composition of the background electrolyte for minimising the …

B Verzola, C Gelfi, PG Righetti - Journal of Chromatography a, 2000 - Elsevier
A novel method is reported for quantifying protein adsorption to naked silica tubings and for
assessing the efficacy of amino quenchers added to the background electrolyte. It consists of …

Surface deactivation in protein and peptide analysis by capillary electrophoresis

I Rodriguez, SFY Li - Analytica chimica acta, 1999 - Elsevier
For the analysis of proteins and peptides in capillary electrophoresis, shielding of the
analytes from the active sites of the fused silica capillary wall is often necessary. There are …

Double-chained surfactants for semipermanent wall coatings in capillary electrophoresis

JE Melanson, NE Baryla, CA Lucy - Analytical chemistry, 2000 - ACS Publications
The double-chained cationic surfactant didodecyldimethylammonium bromide (DDAB) was
found to form more stable coatings onto the walls of CE capillaries than similar single …

Platinum group metallodrug‐protein binding studies by capillary electrophoresis–inductively coupled plasma‐mass spectrometry: A further insight into the reactivity of …

K Polec‐Pawlak, JK Abramski, O Semenova… - …, 2006 - Wiley Online Library
Biochemical speciation analysis has become a hot area of CE research due largely to
growing emergence of inductively coupled plasma (ICP)‐MS as a proper detection …

Buffer additives other than the surfactant sodium dodecyl sulfate for protein separations by capillary electrophoresis

D Corradini - Journal of Chromatography B: Biomedical Sciences …, 1997 - Elsevier
The different compounds utilized as additives to the electrolyte solutions employed in protein
capillary zone electrophoresis (CZE) for minimizing protein-capillary wall interactions, for …