Non-covalent capillary coatings for protein separations in capillary electrophoresis
CA Lucy, AM MacDonald, MD Gulcev - Journal of Chromatography A, 2008 - Elsevier
Adsorption of proteins, particularly basic proteins onto fused silica capillaries severely
degrades capillary electrophoretic performance. This review provides a synopsis of the …
degrades capillary electrophoretic performance. This review provides a synopsis of the …
Microchannel wall coatings for protein separations by capillary and chip electrophoresis
EAS Doherty, RJ Meagher, MN Albarghouthi… - …, 2003 - Wiley Online Library
The necessity for microchannel wall coatings in capillary and chip‐based electrophoretic
analysis of biomolecules is well understood. The regulation or elimination of EOF and the …
analysis of biomolecules is well understood. The regulation or elimination of EOF and the …
The state of the art of dynamic coatings
PG Righetti, C Gelfi, B Verzola, L Castelletti - Electrophoresis, 2001 - Wiley Online Library
The present review highlights the mechanisms of action and efficiency of three major
classes of dynamic coatings so far adopted in capillary electrophoresis:(i) amines to oligo …
classes of dynamic coatings so far adopted in capillary electrophoresis:(i) amines to oligo …
Protein attachment onto silica surfaces–a survey of molecular fundamentals, resulting effects and novel preventive strategies in CE
H Stutz - Electrophoresis, 2009 - Wiley Online Library
This review addresses the fundamentals governing the adsorption of individual protein
molecules onto the surface of fused‐silica capillaries, the protein aggregation to adsorbate …
molecules onto the surface of fused‐silica capillaries, the protein aggregation to adsorbate …
Strategies for capillary electrophoresis: method development and validation for pharmaceutical and biological applications
H Wätzig, M Degenhardt, A Kunkel - Electrophoresis, 1998 - Wiley Online Library
This review is in support of the development of selective, reproducible and validated
capillary electrophoretis (CE) methods. Focusing on pharmaceutical and biological …
capillary electrophoretis (CE) methods. Focusing on pharmaceutical and biological …
Protein adsorption to the bare silica wall in capillary electrophoresis: Quantitative study on the chemical composition of the background electrolyte for minimising the …
B Verzola, C Gelfi, PG Righetti - Journal of Chromatography a, 2000 - Elsevier
A novel method is reported for quantifying protein adsorption to naked silica tubings and for
assessing the efficacy of amino quenchers added to the background electrolyte. It consists of …
assessing the efficacy of amino quenchers added to the background electrolyte. It consists of …
Surface deactivation in protein and peptide analysis by capillary electrophoresis
I Rodriguez, SFY Li - Analytica chimica acta, 1999 - Elsevier
For the analysis of proteins and peptides in capillary electrophoresis, shielding of the
analytes from the active sites of the fused silica capillary wall is often necessary. There are …
analytes from the active sites of the fused silica capillary wall is often necessary. There are …
Double-chained surfactants for semipermanent wall coatings in capillary electrophoresis
JE Melanson, NE Baryla, CA Lucy - Analytical chemistry, 2000 - ACS Publications
The double-chained cationic surfactant didodecyldimethylammonium bromide (DDAB) was
found to form more stable coatings onto the walls of CE capillaries than similar single …
found to form more stable coatings onto the walls of CE capillaries than similar single …
Platinum group metallodrug‐protein binding studies by capillary electrophoresis–inductively coupled plasma‐mass spectrometry: A further insight into the reactivity of …
K Polec‐Pawlak, JK Abramski, O Semenova… - …, 2006 - Wiley Online Library
Biochemical speciation analysis has become a hot area of CE research due largely to
growing emergence of inductively coupled plasma (ICP)‐MS as a proper detection …
growing emergence of inductively coupled plasma (ICP)‐MS as a proper detection …
Buffer additives other than the surfactant sodium dodecyl sulfate for protein separations by capillary electrophoresis
D Corradini - Journal of Chromatography B: Biomedical Sciences …, 1997 - Elsevier
The different compounds utilized as additives to the electrolyte solutions employed in protein
capillary zone electrophoresis (CZE) for minimizing protein-capillary wall interactions, for …
capillary zone electrophoresis (CZE) for minimizing protein-capillary wall interactions, for …