A decade of advances in transposon-insertion sequencing
It has been 10 years since the introduction of modern transposon-insertion sequencing (TIS)
methods, which combine genome-wide transposon mutagenesis with high-throughput …
methods, which combine genome-wide transposon mutagenesis with high-throughput …
Dual RNA-seq of pathogen and host
AJ Westermann, SA Gorski, J Vogel - Nature Reviews Microbiology, 2012 - nature.com
A comprehensive understanding of host–pathogen interactions requires a knowledge of the
associated gene expression changes in both the pathogen and the host. Traditional, probe …
associated gene expression changes in both the pathogen and the host. Traditional, probe …
[HTML][HTML] Functional profiling of a Plasmodium genome reveals an abundance of essential genes
The genomes of malaria parasites contain many genes of unknown function. To assist drug
development through the identification of essential genes and pathways, we have measured …
development through the identification of essential genes and pathways, we have measured …
DEG 15, an update of the Database of Essential Genes that includes built-in analysis tools
Essential genes refer to genes that are required by an organism to survive under specific
conditions. Studies of the minimal-gene-set for bacteria have elucidated fundamental …
conditions. Studies of the minimal-gene-set for bacteria have elucidated fundamental …
The Essential Genome of Escherichia coli K-12
Transposon-directed insertion site sequencing (TraDIS) is a high-throughput method
coupling transposon mutagenesis with short-fragment DNA sequencing. It is commonly used …
coupling transposon mutagenesis with short-fragment DNA sequencing. It is commonly used …
DEG 10, an update of the database of essential genes that includes both protein-coding genes and noncoding genomic elements
The combination of high-density transposon-mediated mutagenesis and high-throughput
sequencing has led to significant advancements in research on essential genes, resulting in …
sequencing has led to significant advancements in research on essential genes, resulting in …
Pooled CRISPR interference screening enables genome-scale functional genomics study in bacteria with superior performance
To fully exploit the microbial genome resources, a high-throughput experimental platform is
needed to associate genes with phenotypes at the genome level. We present here a novel …
needed to associate genes with phenotypes at the genome level. We present here a novel …
In vivo cleavage map illuminates the central role of RNase E in coding and non-coding RNA pathways
Understanding RNA processing and turnover requires knowledge of cleavages by major
endoribonucleases within a living cell. We have employed TIER-seq (transiently inactivating …
endoribonucleases within a living cell. We have employed TIER-seq (transiently inactivating …
[HTML][HTML] Tryptophan biosynthesis protects mycobacteria from CD4 T-cell-mediated killing
Bacteria that cause disease rely on their ability to counteract and overcome host defenses.
Here, we present a genome-scale study of Mycobacterium tuberculosis (Mtb) that uncovers …
Here, we present a genome-scale study of Mycobacterium tuberculosis (Mtb) that uncovers …
The design and analysis of transposon insertion sequencing experiments
Transposon insertion sequencing (TIS) is a powerful approach that can be extensively
applied to the genome-wide definition of loci that are required for bacterial growth under …
applied to the genome-wide definition of loci that are required for bacterial growth under …