Adoptive transfer of chimeric antigen receptor (CAR) T lymphocytes is a powerful technology
that has revolutionized the way we conceive immunotherapy. The impressive clinical results …

Transposons are mobile genetic elements evolved to execute highly efficient integration of
their genes into the genomes of their host cells. These natural DNA transfer vehicles have …

Immunotherapy with T cell modified with gamma-retroviral or lentiviral (LV) vectors to
express a chimeric antigen receptor (CAR) has shown remarkable efficacy in clinical trials …

Transposons have provided important genetic tools for functional genomic screens in lower
eukaryotes but have proven less useful in higher eukaryotes because of their low …

Light-mediated control of protein–protein interactions to regulate cellular pathways is an
important application of optogenetics. Here, we report an optogenetic system based on the …

Cell line development represents a crucial step in the development process of a therapeutic
glycoprotein. Chinese hamster ovary (CHO) cells are the most frequently employed …

Abstract The Slee** Beauty (SB) transposon system, derived from teleost fish sequences,
is extremely effective at delivering DNA to vertebrate genomes, including those of humans …

Viruses and transposons are efficient tools for permanently delivering foreign DNA into
vertebrate genomes but exhibit diminished activity when cargo exceeds 8 kilobases (kb) …

The widespread clinical implementation of gene therapy requires the ability to stably
integrate genetic information through gene transfer vectors in a safe, effective, and …

Genetic modification of clinical-grade T cells is undertaken to augment function, including
redirecting specificity for desired antigen. We and others have introduced a chimeric antigen …