Data-independent acquisition (DIA) mass spectrometry (MS) has emerged as a powerful
technology for high-throughput, accurate, and reproducible quantitative proteomics. This …

The data‐independent acquisition mass spectrometry (DIA‐MS) has rapidly evolved as a
powerful alternative for highly reproducible proteome profiling with a unique strength of …

Adjuvant chemotherapy benefits patients with resected pancreatic ductal adenocarcinoma
(PDAC), but the compromised physical state of post-operative patients can hinder …

Sperm motility is crucial for successful fertilization. Highly decorated doublet microtubules
(DMTs) form the sperm tail skeleton, which propels the movement of spermatozoa. Using …

Proteomics, the study of all the proteins in biological systems, is becoming a data‐rich
science. Protein sequences and structures are comprehensively catalogued in online …

Amyloid-β, tau pathology, and biomarkers of neurodegeneration make up the core
diagnostic biomarkers of Alzheimer disease (AD). However, these proteins represent only a …

High-throughput lysis and proteolytic digestion of biopsy-level tissue specimens is a major
bottleneck for clinical proteomics. Here we describe a detailed protocol of pressure cycling …

Phosphoproteomics can provide insights into cellular signaling dynamics. To achieve deep
and robust quantitative phosphoproteomics profiling for minute amounts of sample, we here …

Background: Early screening for colorectal cancer (CRC) is essential to improve its
prognosis. Liquid biopsies are increasingly being considered for diagnosing cancer due to …

INTRODUCTION Precursor messenger RNA (pre-mRNA) splicing, which involves the
removal of noncoding introns and the ligation of the coding exons, is achieved by the …